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CoraLite® Plus 647-conjugated EED Monoclonal antibody

EED Monoclonal Antibody for IF-P, FC (Intra)

Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse

Applications

IF-P, FC (Intra)

Conjugate

CoraLite® Plus 647 Fluorescent Dye

CloneNo.

1D11D4

Cat no : CL647-67756

Print datasheet

Synonyms

WAIT1, WAIT 1, Polycomb protein EED, Embryonic ectoderm development protein, embryonic ectoderm development

验证数据展示

  • IF Staining of mouse testis using CL647-67756

    IF Staining of mouse testis using CL647-67756

    Immunofluorescent analysis of (4% PFA) fixed mouse testis tissue using CoraLite® Plus 647 EED antibody (CL647-67756, Clone: 1D11D4 ) at dilution of 1:200.

  • IF Staining of mouse testis using CL647-67756

    IF Staining of mouse testis using CL647-67756

    Immunofluorescent analysis of (4% PFA) fixed mouse testis tissue using CoraLite® Plus 647 EED antibody (CL647-67756, Clone: 1D11D4 ) at dilution of 1:200.

  • FC experiment of HeLa using CL647-67756

    FC experiment of HeLa using CL647-67756

    1x10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite® Plus 647-conjugated EED Monoclonal antibody (CL647-67756, Clone:1D11D4)(red), or 0.4 ug CoraLite® Plus 647 Mouse IgG2b isotype control (11B8C4) (CL647-66360-3, Clone: 11B8C4) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

经过测试的应用

Positive IF-P detected inmouse testis tissue
Positive FC (Intra) detected inHeLa cells
For other applications, we recommend the unconjugated version of this antibody, 67756-1-Ig

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)-PIF-P : 1:300-1:1200
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL647-67756 targets EED in IF-P, FC (Intra) applications and shows reactivity with human, mouse samples.

Tested Applications IF-P, FC (Intra) Application Description
Tested Reactivity human, mouse
Immunogen EED fusion protein Ag10369 种属同源性预测
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Full Name embryonic ectoderm development
Synonyms WAIT1, WAIT 1, Polycomb protein EED, Embryonic ectoderm development protein, embryonic ectoderm development
Calculated Molecular Weight 466 aa, 53 kDa
GenBank Accession NumberBC068995
Gene Symbol EED
Gene ID (NCBI) 8726
RRIDAB_2920305
Conjugate CoraLite® Plus 647 Fluorescent Dye
Excitation/Emission Maxima Wavelengths654 nm / 674 nm
Form Liquid
Purification MethodProtein A purification
UNIPROT IDO75530
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

EED is a member of the Polycomb-group (PcG) family. PcG family members form multimeric protein complexes, which are involved in maintaining the transcriptional repressive state of genes over successive cell generations. This protein interacts with enhancer of zeste 2, the cytoplasmic tail of integrin beta7, immunodeficiency virus type 1 (HIV-1) MA protein, and histone deacetylase proteins. This protein mediates repression of gene activity through histone deacetylation, and may act as a specific regulator of integrin function. EED has three isoforms, with the molecular weight of isoforms 1/2/3: 50/53/46 kDa.

实验方案

Product Specific Protocols
IF protocol for CL Plus 647 EED antibody CL647-67756Download protocol
FC protocol for CL Plus 647 EED antibody CL647-67756Download protocol
Standard Protocols
Click here to view our Standard Protocols