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验证数据展示

  • IF Staining of mouse testis using CL488-67756

    IF Staining of mouse testis using CL488-67756

    Immunofluorescent analysis of (4% PFA) fixed mouse testis tissue using CoraLite® Plus 488 EED antibody (CL488-67756, Clone: 1D11D4 ) at dilution of 1:200.

  • IF Staining of mouse testis using CL488-67756

    IF Staining of mouse testis using CL488-67756

    Immunofluorescent analysis of (4% PFA) fixed mouse testis tissue using CoraLite® Plus 488 EED antibody (CL488-67756, Clone: 1D11D4 ) at dilution of 1:200.

  • FC experiment of HeLa using CL488-67756

    FC experiment of HeLa using CL488-67756

    1X10^6 HeLa cells were intracellularly stained with 0.2 ug CoraLite® Plus 488 Anti-Human EED (CL488-67756, Clone:1D11D4) (red), or 0.2 ug Mouse IgG2b Isotype Control (CL488-66360-3, Clone: K11B8C4B5) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

CoraLite® Plus 488-conjugated EED Monoclonal antibody

EED Monoclonal Antibody for IF-P, FC (Intra)
Cat No. CL488-67756

产品说明书

CloneNo. 1D11D4

宿主/亚型

Mouse / IgG2b

种属反应性

human, mouse

应用

IF-P, FC (Intra)

别名

WAIT1, WAIT 1, Polycomb protein EED, Embryonic ectoderm development protein, embryonic ectoderm development

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive IF-P detected inmouse testis tissue
Positive FC (Intra) detected inHeLa cells
For other applications, we recommend the unconjugated version of this antibody, 67756-1-Ig

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)-PIF-P : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-67756 targets EED in IF-P, FC (Intra) applications and shows reactivity with human, mouse samples.

经测试应用 IF-P, FC (Intra) Application Description
经测试反应性 human, mouse
免疫原 EED fusion protein Ag10369 种属同源性预测
宿主/亚型 Mouse / IgG2b
抗体类别 Monoclonal
产品类型 Antibody
全称 embryonic ectoderm development
别名 WAIT1, WAIT 1, Polycomb protein EED, Embryonic ectoderm development protein, embryonic ectoderm development
计算分子量 466 aa, 53 kDa
GenBank蛋白编号BC068995
基因名称 EED
Gene ID (NCBI) 8726
RRIDAB_2919539
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式 Liquid
纯化方式Protein A purification
UNIPROT IDO75530
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

EED is a member of the Polycomb-group (PcG) family. PcG family members form multimeric protein complexes, which are involved in maintaining the transcriptional repressive state of genes over successive cell generations. This protein interacts with enhancer of zeste 2, the cytoplasmic tail of integrin beta7, immunodeficiency virus type 1 (HIV-1) MA protein, and histone deacetylase proteins. This protein mediates repression of gene activity through histone deacetylation, and may act as a specific regulator of integrin function. EED has three isoforms, with the molecular weight of isoforms 1/2/3: 50/53/46 kDa.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 EED antibody CL488-67756Download protocol
FC protocol for CL Plus 488 EED antibody CL488-67756Download protocol
Standard Protocols
Click here to view our Standard Protocols