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Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis using 83264-6-RR (same clone as 83264-6-PBS)

    WB analysis using 83264-6-RR (same clone as 83264-6-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83264-6-RR (EFHD2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83264-6-PBS in a different storage buffer formulation.

  • FC experiment of A431 using 83264-6-RR (same clone as 83264-6-PBS)

    FC experiment of A431 using 83264-6-RR (same clone as 83264-6-PBS)

    1x10^6 A431 cells were intracellularly stained with 0.25 ug EFHD2 Recombinant antibody (83264-6-RR, Clone:240185A8) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83264-6-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 83264-6-RR

    Affinity and Kinetic Characterization of 83264-6-RR

    Biolayer interferometry (BLl) kinetic assays of 83264-6-RR against Human EFHD2 were performed. The affinity constant is 4.63 nM.

EFHD2 Recombinant antibody, PBS Only

EFHD2 Uni-rAbTM Recombinant Antibody for WB, FC (Intra), ELISA
Cat No. 83264-6-PBS

产品说明书

CloneNo. 240185A8

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, FC (Intra), ELISA

别名

SWS1, Swiprosin-1, Swiprosin 1, EF-hand domain-containing protein D2, 240185A8

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy EFHD2 Ready-To-Use IHC Kit. EFHD2 primary antibody included.

产品信息

83264-6-PBS targets EFHD2 in WB, FC (Intra), ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, FC (Intra), ELISA Application Description
经测试反应性 human, mouse
免疫原 EFHD2 fusion protein Ag35084 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 EF-hand domain family, member D2
别名 SWS1, Swiprosin-1, Swiprosin 1, EF-hand domain-containing protein D2, 240185A8
计算分子量27 kDa
观测分子量27 kDa
GenBank蛋白编号BC023611
基因名称 EFHD2
Gene ID (NCBI) 79180
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDQ96C19
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

EFHD2 also named Swiprosin-1, is a Ca2+ binding actin protein. The expression of EFHD2 is upregulated during the activation of immune cells, epithelial and endothelial cells. The expression of EFHD2 is regulated by diverse signaling pathways that are contingent upon the specific type of cells.