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Species Reactivity
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验证数据展示

  • WB analysis using 83264-6-RR

    WB analysis using 83264-6-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83264-6-RR (EFHD2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • FC experiment of A431 using 83264-6-RR

    FC experiment of A431 using 83264-6-RR

    1x10^6 A431 cells were intracellularly stained with 0.25 ug EFHD2 Recombinant antibody (83264-6-RR, Clone:240185A8) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Affinity and Kinetic Characterization of 83264-6-RR

    Affinity and Kinetic Characterization of 83264-6-RR

    Biolayer interferometry (BLl) kinetic assays of 83264-6-RR against Human EFHD2 were performed. The affinity constant is 4.63 nM.

EFHD2 Recombinant antibody

EFHD2 Uni-rAbTM Recombinant Antibody for WB, FC (Intra), ELISA
Cat No. 83264-6-RR

Print datasheet

CloneNo. 240185A8

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, FC (Intra), ELISA

Synonyms

SWS1, Swiprosin-1, Swiprosin 1, EF-hand domain-containing protein D2, 240185A8

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size: 

-/ -

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经过测试的应用

Positive WB detected inHuH-7 cells, NK-92 cells, A549 cells, NCI-H1299 cells, mouse brain tissue
Positive FC (Intra) detected inA431 cells
Planning an IHC experiment? We recommend our IHCeasy EFHD2 Ready-To-Use IHC Kit. EFHD2 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

83264-6-RR targets EFHD2 in WB, FC (Intra), ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, FC (Intra), ELISA Application Description
Tested Reactivity human, mouse
Immunogen EFHD2 fusion protein Ag35084 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name EF-hand domain family, member D2
Synonyms SWS1, Swiprosin-1, Swiprosin 1, EF-hand domain-containing protein D2, 240185A8
Calculated Molecular Weight27 kDa
Observed Molecular Weight27 kDa
GenBank Accession NumberBC023611
Gene Symbol EFHD2
Gene ID (NCBI) 79180
RRIDAB_3670936
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDQ96C19
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

EFHD2 also named Swiprosin-1, is a Ca2+ binding actin protein. The expression of EFHD2 is upregulated during the activation of immune cells, epithelial and endothelial cells. The expression of EFHD2 is regulated by diverse signaling pathways that are contingent upon the specific type of cells.

实验方案

Product Specific Protocols
WB protocol for EFHD2 antibody 83264-6-RRDownload protocol
FC protocol for EFHD2 antibody 83264-6-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols