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验证数据展示

  • WB analysis using 83264-6-RR

    WB analysis using 83264-6-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83264-6-RR (EFHD2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • FC experiment of A431 using 83264-6-RR

    FC experiment of A431 using 83264-6-RR

    1x10^6 A431 cells were intracellularly stained with 0.25 ug EFHD2 Recombinant antibody (83264-6-RR, Clone:240185A8) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Affinity and Kinetic Characterization of 83264-6-RR

    Affinity and Kinetic Characterization of 83264-6-RR

    Biolayer interferometry (BLl) kinetic assays of 83264-6-RR against Human EFHD2 were performed. The affinity constant is 4.63 nM.

EFHD2 Recombinant antibody

EFHD2 Uni-rAbTM Recombinant Antibody for WB, FC (Intra), ELISA
Cat No. 83264-6-RR

产品说明书

CloneNo. 240185A8

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, FC (Intra), ELISA

别名

SWS1, Swiprosin-1, Swiprosin 1, EF-hand domain-containing protein D2, 240185A8

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHuH-7 cells, NK-92 cells, A549 cells, NCI-H1299 cells, mouse brain tissue
Positive FC (Intra) detected inA431 cells
Planning an IHC experiment? We recommend our IHCeasy EFHD2 Ready-To-Use IHC Kit. EFHD2 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:10000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

83264-6-RR targets EFHD2 in WB, FC (Intra), ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, FC (Intra), ELISA Application Description
经测试反应性 human, mouse
免疫原 EFHD2 fusion protein Ag35084 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 EF-hand domain family, member D2
别名 SWS1, Swiprosin-1, Swiprosin 1, EF-hand domain-containing protein D2, 240185A8
计算分子量27 kDa
观测分子量27 kDa
GenBank蛋白编号BC023611
基因名称 EFHD2
Gene ID (NCBI) 79180
RRIDAB_3670936
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDQ96C19
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

EFHD2 also named Swiprosin-1, is a Ca2+ binding actin protein. The expression of EFHD2 is upregulated during the activation of immune cells, epithelial and endothelial cells. The expression of EFHD2 is regulated by diverse signaling pathways that are contingent upon the specific type of cells.

实验方案

Product Specific Protocols
WB protocol for EFHD2 antibody 83264-6-RRDownload protocol
FC protocol for EFHD2 antibody 83264-6-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols