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验证数据展示

  • IF Staining of HaCaT using 68643-1-Ig

    IF Staining of HaCaT using 68643-1-Ig

    Immunofluorescent analysis of un-fixed HaCaT cells using EGFR antibody (68643-1-Ig, Clone: 2C8C12 ) at dilution of 1:1000 and Multi-rAb CoraLite® Plus 594-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (Cat.NO. RGAM004 ).

  • WB analysis of SCaBER using 68643-1-Ig

    WB analysis of SCaBER using 68643-1-Ig

    SCaBER cells were subjected to SDS PAGE followed by western blot with 68643-1-Ig (EGFR antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Blocking experiment of HeLa cells using 68643-1-Ig

    Blocking experiment of HeLa cells using 68643-1-Ig

    Human EGF (Cat.NO. HZ-1326 ) stimulates proliferation of HeLa cells in a dose-dependent manner (blue curve, refer to bottom X-left Y axis). The activity of human EGF (Cat.NO. HZ-1326 ) is blocked by mouse anti-human EGFR monoclonal antibody 68643-1-Ig at serial dose (red curve, refer to top X-right Y axis). The EC50 is typically 0.2-0.8 μg/mL at the presence of 1ng/ml EGF (Cat.NO. HZ-1326 ).

  • FC experiment of A431 using 68643-1-Ig

    FC experiment of A431 using 68643-1-Ig

    1x10^6 A431 cells were surface stained with 0.2 μg EGFR Monoclonal Antibody (68643-1-Ig, Clone:2C8C12) and CoraLite488-conjugated Goat Anti-Mouse IgG(H+L) (Cat.NO. SA00013-1)(red), or 0.2 μg Mouse IgG1 Isotype Control (66360-1-Ig, Clone: T1F8D3F10) (blue). Cells were not fixed.

EGFR Monoclonal antibody

EGFR Monoclonal Antibody for WB, IF/ICC, FC, ELISA, Blocking
Cat No. 68643-1-Ig

产品说明书

CloneNo. 2C8C12

宿主/亚型

Mouse / IgG1

种属反应性

human

应用

WB, IF/ICC, FC, ELISA, Blocking

别名

Proto-oncogene c-ErbB-1, Epidermal growth factor receptor, EGF Receptor , EGF R, 2C8C12

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inSCaBER cells, HaCaT cells, MDA-MB-468 cells, A431 cells
Positive IF/ICC detected inHaCaT cells, A431 cells
Positive FC detected inA431 cells
Positive Blocking detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy EGFR Ready-To-Use IHC Kit. EGFR primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:500-1:2000
Flow Cytometry (FC)FC : 0.20 ug per 10^6 cells in a 100 µl suspension
BLOCKINGBLOCKING : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68643-1-Ig targets EGFR in WB, IF/ICC, FC, ELISA, Blocking applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, FC, ELISA, Blocking Application Description
经测试反应性 human
免疫原 EGFR fusion protein Ag24947 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 epidermal growth factor receptor (erythroblastic leukemia viral (v-erb-b) oncogene homolog, avian)
别名 Proto-oncogene c-ErbB-1, Epidermal growth factor receptor, EGF Receptor , EGF R, 2C8C12
计算分子量134kd
观测分子量160 kDa
GenBank蛋白编号NM_005228.5
基因名称 EGFR
Gene ID (NCBI) 1956
RRIDAB_3670387
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein G purification
UNIPROT IDP00533
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

EGFR, also named ERBB1, is a cell-surface receptor for members of the epidermal growth factor family (EGF-family) of extracellular protein ligands. Binding of the protein to a ligand induces receptor dimerization and tyrosine autophosphorylation and leads to cell proliferation. The gene resides on chromosome 7p12, encoding a 170 kDa membrane-associated glycoprotein. Recent studies have shown EGFR plays a critical role in cancer development and progression, including cell proliferation, apoptosis, angiogenesis, and metastatic spread. Mutations in this gene are associated with lung cancer. When stained with live cells, EGFR can be transported into the cell interior, which is consistent with the literature report (PMID: 18084617).

实验方案

Product Specific Protocols
WB protocol for EGFR antibody 68643-1-IgDownload protocol
IF protocol for EGFR antibody 68643-1-IgDownload protocol
FC protocol for EGFR antibody 68643-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols