• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • IF Staining of HaCaT using 68643-1-Ig (same clone as 68643-1-PBS)

    IF Staining of HaCaT using 68643-1-Ig (same clone as 68643-1-PBS)

    Immunofluorescent analysis of un-fixed HaCaT cells using EGFR antibody (68643-1-Ig, Clone: 2C8C12 ) at dilution of 1:1000 and Multi-rAb CoraLite® Plus 594-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (Cat.NO. RGAM004 ). This data was developed using the same antibody clone with 68643-1-PBS in a different storage buffer formulation.

  • WB analysis of SCaBER using 68643-1-Ig (same clone as 68643-1-PBS)

    WB analysis of SCaBER using 68643-1-Ig (same clone as 68643-1-PBS)

    SCaBER cells were subjected to SDS PAGE followed by western blot with 68643-1-Ig (EGFR antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68643-1-PBS in a different storage buffer formulation.

  • Blocking experiment of HeLa cells using 68643-1-Ig (same clone as 68643-1-PBS)

    Blocking experiment of HeLa cells using 68643-1-Ig (same clone as 68643-1-PBS)

    Human EGF (Cat.NO. HZ-1326 ) stimulates proliferation of HeLa cells in a dose-dependent manner (blue curve, refer to bottom X-left Y axis). The activity of human EGF (Cat.NO. HZ-1326 ) is blocked by mouse anti-human EGFR monoclonal antibody 68643-1-Ig at serial dose (red curve, refer to top X-right Y axis). The EC50 is typically 0.2-0.8 μg/mL at the presence of 1ng/ml EGF (Cat.NO. HZ-1326 ). This data was developed using the same antibody clone with 68643-1-PBS in a different storage buffer formulation.

  • FC experiment of A431 using 68643-1-Ig (same clone as 68643-1-PBS)

    FC experiment of A431 using 68643-1-Ig (same clone as 68643-1-PBS)

    1x10^6 A431 cells were surface stained with 0.2 μg EGFR Monoclonal Antibody (68643-1-Ig, Clone:2C8C12) and CoraLite488-conjugated Goat Anti-Mouse IgG(H+L) (Cat.NO. SA00013-1)(red), or 0.2 μg Mouse IgG1 Isotype Control (66360-1-Ig, Clone: T1F8D3F10) (blue). Cells were not fixed. This data was developed using the same antibody clone with 68643-1-PBS in a different storage buffer formulation.

  • Sandwich ELISA standard curve of MP50069-1

    Sandwich ELISA standard curve of MP50069-1

    Sandwich ELISA standard curve of MP50069-1, EGFR Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 68643-1-PBS. Detection antibody: 68643-2-PBS. Standard: Eg0204. Range: 0.156-10 ng/mL.

EGFR Monoclonal antibody, PBS Only (Capture)

EGFR Monoclonal Antibody for WB, IF/ICC, FC, Sandwich ELISA, Indirect ELISA, Blocking, Sample test
Cat No. 68643-1-PBS

产品说明书

CloneNo. 2C8C12

宿主/亚型

Mouse / IgG1

种属反应性

human

应用

WB, IF/ICC, FC, Sandwich ELISA, Indirect ELISA, Blocking, Sample test

别名

Proto-oncogene c-ErbB-1, Epidermal growth factor receptor, EGF Receptor , EGF R, 2C8C12

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

点击咨询售前客服

点击咨询售后客服

Planning an IHC experiment? We recommend our IHCeasy EGFR Ready-To-Use IHC Kit. EGFR primary antibody included.

产品信息

68643-1-PBS targets EGFR as part of a matched antibody pair:

MP50069-1: 68643-1-PBS capture and 68643-2-PBS detection (validated in Sandwich ELISA)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 WB, IF/ICC, FC, Sandwich ELISA, Indirect ELISA, Blocking, Sample test Application Description
经测试反应性 human
免疫原 EGFR fusion protein Ag24947 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 epidermal growth factor receptor (erythroblastic leukemia viral (v-erb-b) oncogene homolog, avian)
别名 Proto-oncogene c-ErbB-1, Epidermal growth factor receptor, EGF Receptor , EGF R, 2C8C12
计算分子量134kd
观测分子量160 kDa
GenBank蛋白编号NM_005228.5
基因名称 EGFR
Gene ID (NCBI) 1956
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein G purification
UNIPROT IDP00533
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

EGFR, also named ERBB1, is a cell-surface receptor for members of the epidermal growth factor family (EGF-family) of extracellular protein ligands. Binding of the protein to a ligand induces receptor dimerization and tyrosine autophosphorylation and leads to cell proliferation. The gene resides on chromosome 7p12, encoding a 170 kDa membrane-associated glycoprotein. Recent studies have shown EGFR plays a critical role in cancer development and progression, including cell proliferation, apoptosis, angiogenesis, and metastatic spread. Mutations in this gene are associated with lung cancer. When stained with live cells, EGFR can be transported into the cell interior, which is consistent with the literature report (PMID: 18084617).