ENO3 Monoclonal antibody
ENO3 Monoclonal Antibody for WB, IF/ICC, ELISA
Host / Isotype
Mouse / IgG2b
Reactivity
Human, Mouse, Rat, Pig
Applications
WB, IF/ICC, ELISA
Conjugate
Unconjugated
CloneNo.
2C12G3
验证数据展示
经过测试的应用
Positive WB detected in | HeLa cells, HEK-293 cells, HepG2 cells, Jurkat cells, pig skeletal muscle tissue, rabbit skeletal muscle tissue, rat skeletal muscle tissue, mouse skeletal muscle tissue, mouse brain tissue |
Positive IF/ICC detected in | HeLa cells |
Planning an IHC experiment? We recommend our IHCeasy ENO3 Ready-To-Use IHC Kit. ENO3 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:5000-1:50000 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
68147-1-Ig targets ENO3 in WB, IF/ICC, ELISA applications and shows reactivity with Human, Mouse, Rat, Pig samples.
Tested Applications | WB, IF/ICC, ELISA Application Description |
Tested Reactivity | Human, Mouse, Rat, Pig |
Immunogen | ENO3 fusion protein Ag9642 种属同源性预测 |
Host / Isotype | Mouse / IgG2b |
Class | Monoclonal |
Type | Antibody |
Full Name | enolase 3 (beta, muscle) |
Synonyms | Beta enolase, ENO3, Enolase 3, enolase 3 (beta, muscle), MSE, Muscle specific enolase, Skeletal muscle enolase |
Calculated Molecular Weight | 434 aa, 47 kDa |
Observed Molecular Weight | 42-47 kDa |
GenBank Accession Number | BC017249 |
Gene Symbol | ENO3 |
Gene ID (NCBI) | 2027 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purification |
UNIPROT ID | P13929 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Enolase 3 (ENO3) encodes the β-subunit of enolase, which is distributed in various tissues, including liver, lung, skeletal, and heart. ENO3 plays an important role in both glycogen and cholesterol metabolism. It has been demonstrated that ENO3 deficiency may lead to metabolic myopathies, and ENO3 speeds up hepatic cholesterol ester cumulation induced via the mediation of cholesteryl ester generation.Apart from these, ENO3 has also been involved in various tumors, though the results are contradictory. For example, ENO3 is up-regulated in STK11 mutant pulmonary carcinoma and colorectal carcinoma, while down-regulated in pancreatic cancer and hepatocellular carcinomas. ENO3 has 3 isoforms with the molecular mass of 42-47 kDa. (PMID: 33987359, 35004693)
实验方案
Product Specific Protocols | |
---|---|
WB protocol for ENO3 antibody 68147-1-Ig | Download protocol |
IF protocol for ENO3 antibody 68147-1-Ig | Download protocol |
Standard Protocols | |
---|---|
Click here to view our Standard Protocols |