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验证数据展示

  • IF Staining of HeLa using CL488-68147

    IF Staining of HeLa using CL488-68147

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using CoraLite® Plus 488 ENO3 antibody (CL488-68147, Clone: 2C12G3 ) at dilution of 1:200.

CoraLite® Plus 488-conjugated ENO3 Monoclonal antibody

ENO3 Monoclonal Antibody for IF/ICC
Cat No. CL488-68147

Print datasheet

CloneNo. 2C12G3

Host / Isotype

Mouse / IgG2b

Reactivity

Human, Mouse, Rat, Pig

Applications

IF/ICC

Synonyms

Beta enolase, ENO3, Enolase 3, enolase 3 (beta, muscle), MSE, Muscle specific enolase, Skeletal muscle enolase

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite® Plus 488
Size: 

-/ -

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经过测试的应用

Positive IF/ICC detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy ENO3 Ready-To-Use IHC Kit. ENO3 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 68147-1-Ig

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-68147 targets ENO3 in IF/ICC applications and shows reactivity with Human, Mouse, Rat, Pig samples.

Tested Applications IF/ICC Application Description
Tested Reactivity Human, Mouse, Rat, Pig
Immunogen ENO3 fusion protein Ag9642 种属同源性预测
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Full Name enolase 3 (beta, muscle)
Synonyms Beta enolase, ENO3, Enolase 3, enolase 3 (beta, muscle), MSE, Muscle specific enolase, Skeletal muscle enolase
Calculated Molecular Weight 434 aa, 47 kDa
Observed Molecular Weight42-47 kDa
GenBank Accession NumberBC017249
Gene Symbol ENO3
Gene ID (NCBI) 2027
RRIDAB_3084418
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP13929
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Enolase 3 (ENO3) encodes the β-subunit of enolase, which is distributed in various tissues, including liver, lung, skeletal, and heart. ENO3 plays an important role in both glycogen and cholesterol metabolism. It has been demonstrated that ENO3 deficiency may lead to metabolic myopathies, and ENO3 speeds up hepatic cholesterol ester cumulation induced via the mediation of cholesteryl ester generation.Apart from these, ENO3 has also been involved in various tumors, though the results are contradictory. For example, ENO3 is up-regulated in STK11 mutant pulmonary carcinoma and colorectal carcinoma, while down-regulated in pancreatic cancer and hepatocellular carcinomas. ENO3 has 3 isoforms with the molecular mass of 42-47 kDa. (PMID: 33987359, 35004693)

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 ENO3 antibody CL488-68147Download protocol
Standard Protocols
Click here to view our Standard Protocols