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验证数据展示

  • IF Staining of HeLa using CL555-51068

    IF Staining of HeLa using CL555-51068

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CoraLite®555 ERK1/2 antibody (CL555-51068) at dilution of 1:200.

CoraLite®555-conjugated ERK1/2 Polyclonal antibody

ERK1/2 Polyclonal Antibody for IF/ICC
Cat No. CL555-51068

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

IF/ICC

别名

ERK, ERK 2, ERK2, ERT1, MAP kinase 1, MAP kinase 2, MAP kinase isoform p42, MAPK 1, MAPK 2, MAPK1, MAPK2, p38, p40, p41, p41mapk, p42 MAPK, P42MAPK, PRKM1, PRKM2

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite®555
规格: 

-/ -

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经过测试的应用

Positive IF/ICC detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy ERK1/2 Ready-To-Use IHC Kit. ERK1/2 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 51068-1-AP

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL555-51068 targets ERK1/2 in IF/ICC applications and shows reactivity with human, mouse, rat samples.

经测试应用 IF/ICC Application Description
经测试反应性 human, mouse, rat
免疫原 Peptide 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 mitogen-activated protein kinase 1
别名 ERK, ERK 2, ERK2, ERT1, MAP kinase 1, MAP kinase 2, MAP kinase isoform p42, MAPK 1, MAPK 2, MAPK1, MAPK2, p38, p40, p41, p41mapk, p42 MAPK, P42MAPK, PRKM1, PRKM2
计算分子量 360 aa, 41 kDa
观测分子量 38-44 kDa
GenBank蛋白编号BC017832
基因名称 ERK2
Gene ID (NCBI) 5594
RRIDAB_2919654
偶联类型 CoraLite®555 Fluorescent Dye
最大激发/发射波长557 nm / 570nm
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP28482
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ERK1 and ERK2 belongs to the protein kinase superfamily. It is involved in both the initiation and regulation of meiosis, mitosis, and postmitotic functions in differentiated cells by phosphorylating a number of transcription factors such as ELK-1. ERK1/2 catalized the reaction: ATP + a protein = ADP + a phosphoprotein. It is activated by tyrosine phosphorylation in response to INS and NGF. This antibody can recognize both ERK1 and ERK2 with the molecular mass of 38-44 kDa.

实验方案

Product Specific Protocols
IF protocol for CL555 ERK1/2 antibody CL555-51068Download protocol
Standard Protocols
Click here to view our Standard Protocols