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CoraLite® Plus 647-conjugated ERK1/2 Polyclonal antibody

ERK1/2 Polyclonal Antibody for FC (Intra), IF/ICC

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

FC (Intra), IF/ICC

Conjugate

CoraLite® Plus 647 Fluorescent Dye

Cat No : CL647-51068

Print datasheet

Synonyms

ERK, ERK 2, ERK2, ERT1, MAP kinase 1, MAP kinase 2, MAP kinase isoform p42, MAPK 1, MAPK 2, MAPK1, MAPK2, p38, p40, p41, p41mapk, p42 MAPK, P42MAPK, PRKM1, PRKM2

验证数据展示

  • IF Staining of HeLa using CL647-51068

    IF Staining of HeLa using CL647-51068

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CoraLite® Plus 647 ERK1/2 antibody (CL647-51068) at dilution of 1:100.

  • FC experiment of HeLa using CL647-51068

    FC experiment of HeLa using CL647-51068

    1X10^6 HeLa cells were intracellularly stained with 0.2 ug CoraLite® Plus 647 Anti-Human ERK1/2 (CL647-51068) (red), or 0.2 ug Control Antibody (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

经过测试的应用

Positive IF detected inHeLa cells
Positive FC detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy ERK1/2 Ready-To-Use IHC Kit. ERK1/2 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 51068-1-AP

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
Flow Cytometry (FC)FC : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL647-51068 targets ERK1/2 in IF, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

Tested Applications FC (Intra), IF/ICC Application Description
Tested Reactivity human, mouse, rat
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name mitogen-activated protein kinase 1
Synonyms ERK, ERK 2, ERK2, ERT1, MAP kinase 1, MAP kinase 2, MAP kinase isoform p42, MAPK 1, MAPK 2, MAPK1, MAPK2, p38, p40, p41, p41mapk, p42 MAPK, P42MAPK, PRKM1, PRKM2
Calculated Molecular Weight 360 aa, 41 kDa
Observed Molecular Weight 38-44 kDa
GenBank Accession NumberBC017832
Gene Symbol MAPK1
Gene ID (NCBI) 5594
Conjugate CoraLite® Plus 647 Fluorescent Dye
Excitation/Emission Maxima Wavelengths654 nm / 674 nm
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP28482
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ERK1 and ERK2 belongs to the protein kinase superfamily. It is involved in both the initiation and regulation of meiosis, mitosis, and postmitotic functions in differentiated cells by phosphorylating a number of transcription factors such as ELK-1. ERK1/2 catalized the reaction: ATP + a protein = ADP + a phosphoprotein. It is activated by tyrosine phosphorylation in response to INS and NGF. This antibody can recognize both ERK1 and ERK2 with the molecular mass of 38-44 kDa.

实验方案

Product Specific Protocols
IF protocol for CL Plus 647 ERK1/2 antibody CL647-51068Download protocol
FC protocol for CL Plus 647 ERK1/2 antibody CL647-51068Download protocol
Standard Protocols
Click here to view our Standard Protocols