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CoraLite® Plus 488-conjugated ESRRB Monoclonal antibody

ESRRB Monoclonal Antibody for IF/ICC

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

IF/ICC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

3E10C1

Cat no : CL488-66818

Print datasheet

Synonyms

验证数据展示

  • IF Staining of Caco-2 using CL488-66818

    IF Staining of Caco-2 using CL488-66818

    Immunofluorescent analysis of (4% PFA) fixed Caco-2 cells using CoraLite® Plus 488 ESRRB antibody (CL488-66818, Clone: 3E10C1 ) at dilution of 1:200, Alpha Tubulin antibody (80762-1-RR, Clone: 1G7, red).

经过测试的应用

Positive IF/ICC detected inCaco-2 cells
Planning an IHC experiment? We recommend our IHCeasy ESRRB Ready-To-Use IHC Kit. ESRRB primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 66818-1-Ig

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-66818 targets ESRRB in IF/ICC applications and shows reactivity with human, mouse, rat samples.

Tested Applications IF/ICC Application Description
Tested Reactivity human, mouse, rat
Immunogen ESRRB fusion protein Ag18626 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name estrogen-related receptor beta
Synonyms
Calculated Molecular Weight 508 aa, 56 kDa
Observed Molecular Weight 47 kDa
GenBank Accession NumberBC131517
Gene Symbol ESRRB
Gene ID (NCBI) 2103
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein G purification
UNIPROT IDO95718
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Estrogen related receptor beta (ESRRB), a NR3 Steroid Receptor, has been shown to affect early placental development. Also it can act as a repressor of transcriptional activity mediated by the glucocorticoid receptor (GR). It binds as a monomer to the extended half-site TNAAGTGCA and as a homodimer to the estrogen response element (ERE), palindromic thyroid hormone response element (TRE(pal)), and SF-1 response element, but not to the glucocorticoid response element (GRE). Estrogen related receptor beta mutations lead to abnormal development of the chorion and defective diploid trophoblast proliferation, and are lethal during midgestation. Expression has been documented in mice in the extra embryonic ectoderm that gives rise to the chorion. ESTs have been isolated from normal human eye, lung, and testis libraries. The ligand for the receptor is PPARgamma coactivator 1 (PGC-1) beta. ESRRB exists various isoforms and molecular weight of each isoform is 48 kDa,55 kDa and 56 kDa.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 ESRRB antibody CL488-66818Download protocol
Standard Protocols
Click here to view our Standard Protocols