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  • Featured Product
  • KD/KO Validated

EXOSC10 Polyclonal antibody

EXOSC10 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Cat no : 16731-1-AP

Print datasheet

Synonyms

Autoantigen PM/Scl 2, EXOSC10, exosome component 10, p2, p3, p4, PM Scl, PM/Scl 100, PMSCL, PMSCL2, RRP6, Rrp6p

验证数据展示

  • WB analysis of HeLa using 16731-1-AP

    WB analysis of HeLa using 16731-1-AP

    WB result of EXOSC10 antibody (16731-1-AP; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-EXOSC10 transfected HeLa cells.

  • WB analysis using 16731-1-AP

    WB analysis using 16731-1-AP

    BxPC-3 cells were subjected to SDS PAGE followed by western blot with 16731-1-AP (EXOSC10 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • IHC staining of human stomach cancer using 16731-1-AP

    IHC staining of human stomach cancer using 16731-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 16731-1-AP (EXOSC10 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with EXOSC10 antibody included
  • IHC staining of human stomach cancer using 16731-1-AP

    IHC staining of human stomach cancer using 16731-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 16731-1-AP (EXOSC10 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with EXOSC10 antibody included
  • IP experiment of HeLa using 16731-1-AP

    IP experiment of HeLa using 16731-1-AP

    IP result of anti-EXOSC10 (IP:16731-1-AP, 4ug; Detection:16731-1-AP 1:700) with HeLa cells lysate 1200ug.

  • IF Staining of HeLa using 16731-1-AP

    IF Staining of HeLa using 16731-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using EXOSC10 antibody (16731-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

经过测试的应用

Positive WB detected inBxPC-3 cells, HeLa cells, mouse spleen tissue
Positive IP detected inHeLa cells
Positive IHC detected inhuman stomach cancer tissue, mouse skin tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy EXOSC10 Ready-To-Use IHC Kit. EXOSC10 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

16731-1-AP targets EXOSC10 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IHC, IF/ICC, IP, ELISA Application Description
Cited ApplicationsWB, IHC
Tested Reactivity human, mouse
Cited Reactivityhuman
Immunogen EXOSC10 fusion protein Ag10189 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name exosome component 10
Synonyms Autoantigen PM/Scl 2, EXOSC10, exosome component 10, p2, p3, p4, PM Scl, PM/Scl 100, PMSCL, PMSCL2, RRP6, Rrp6p
Calculated Molecular Weight 885 aa, 101 kDa
Observed Molecular Weight 100 kDa
GenBank Accession NumberBC073788
Gene Symbol EXOSC10
Gene ID (NCBI) 5394
RRIDAB_2101730
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ01780
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

About 50% of patients with polymyositis/scleroderma (PM-Scl) overlap syndrome are reported to have autoantibodies to a neuclear/nucleolar particle termed PM-Scl. Exosome component 10 (EXOSC10), also named autoantigen PM/Scl 2, is the 100 kDa antigen component of PM-Scl and is recognized by most sera of PM-Scl paitents. EXOSC10 is strongly enriched in the nucleolus and a small amount has been found in cytoplasm supporting the existence of a nucleolar RNA exosome complex form. As a putative catalytic component of the RNA exosome complex which has 3'->5' exoribonuclease activity, EXOSC10 participates in a multitude of cellular RNA processing and degradation events.

实验方案

Product Specific Protocols
WB protocol for EXOSC10 antibody 16731-1-APDownload protocol
IHC protocol for EXOSC10 antibody 16731-1-APDownload protocol
IF protocol for EXOSC10 antibody 16731-1-APDownload protocol
IP protocol for EXOSC10 antibody 16731-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB,IHC

PeerJ

EXOSC10 is a novel hepatocellular carcinoma prognostic biomarker: a comprehensive bioinformatics analysis and experiment verification

Authors - Zhi-Yong Meng
  • KD Validated
View Article