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CoraLite® Plus 488-conjugated EXOSC10 Polyclonal antibody
EXOSC10 Polyclonal Antibody for IF/ICC
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse
Applications
IF/ICC
Conjugate
CoraLite® Plus 488 Fluorescent Dye
验证数据展示
经过测试的应用
Positive IF detected in | HeLa cells |
Planning an IHC experiment? We recommend our IHCeasy EXOSC10 Ready-To-Use IHC Kit. EXOSC10 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 16731-1-AP
推荐稀释比
Application | Dilution |
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Immunofluorescence (IF) | IF : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
CL488-16731 targets EXOSC10 in IF applications and shows reactivity with human, mouse samples.
Tested Applications | IF/ICC Application Description |
Tested Reactivity | human, mouse |
Immunogen | EXOSC10 fusion protein Ag10189 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Full Name | exosome component 10 |
Synonyms | Autoantigen PM/Scl 2, EXOSC10, exosome component 10, p2, p3, p4, PM Scl, PM/Scl 100, PMSCL, PMSCL2, RRP6, Rrp6p |
Calculated Molecular Weight | 885 aa, 101 kDa |
Observed Molecular Weight | 100 kDa |
GenBank Accession Number | BC073788 |
Gene Symbol | EXOSC10 |
Gene ID (NCBI) | 5394 |
Conjugate | CoraLite® Plus 488 Fluorescent Dye |
Excitation/Emission Maxima Wavelengths | 493 nm / 522 nm |
Form | Liquid |
Purification Method | Antigen affinity purification |
UNIPROT ID | Q01780 |
Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
About 50% of patients with polymyositis/scleroderma (PM-Scl) overlap syndrome are reported to have autoantibodies to a neuclear/nucleolar particle termed PM-Scl. Exosome component 10 (EXOSC10), also named autoantigen PM/Scl 2, is the 100 kDa antigen component of PM-Scl and is recognized by most sera of PM-Scl paitents. EXOSC10 is strongly enriched in the nucleolus and a small amount has been found in cytoplasm supporting the existence of a nucleolar RNA exosome complex form. As a putative catalytic component of the RNA exosome complex which has 3'->5' exoribonuclease activity, EXOSC10 participates in a multitude of cellular RNA processing and degradation events.
实验方案
Product Specific Protocols | |
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IF protocol for CL Plus 488 EXOSC10 antibody CL488-16731 | Download protocol |
FC protocol for CL Plus 488 EXOSC10 antibody CL488-16731 | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |