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Host
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Species Reactivity
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Species Reactivity
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Conjugate
0
Conjugate
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Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis using 84346-4-RR (same clone as 84346-4-PBS)

    WB analysis using 84346-4-RR (same clone as 84346-4-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84346-4-RR (EXOSC6 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84346-4-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 84346-4-RR (same clone as 84346-4-PBS)

    IF Staining of HeLa using 84346-4-RR (same clone as 84346-4-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using EXOSC6 antibody (84346-4-RR, Clone: 241535C3 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 84346-4-PBS in a different storage buffer formulation.

  • FC experiment of HeLa using 84346-4-RR (same clone as 84346-4-PBS)

    FC experiment of HeLa using 84346-4-RR (same clone as 84346-4-PBS)

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug EXOSC6 Recombinant antibody (84346-4-RR, Clone:241535C3) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 84346-4-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 84346-4-RR

    Affinity and Kinetic Characterization of 84346-4-RR

    Biolayer interferometry (BLl) kinetic assays of 84346-4-RR against Human EXOSC6 were performed. The affinity constant is below 1 pM.

EXOSC6 Recombinant antibody, PBS Only

EXOSC6 Uni-rAbTM Recombinant Antibody for WB, IF/ICC, FC (Intra), Indirect ELISA
Cat No. 84346-4-PBS

产品说明书

CloneNo. 241535C3

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IF/ICC, FC (Intra), Indirect ELISA

别名

241535C3, EAP4, exosome component 6, hMtr3, hMtr3p

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

84346-4-PBS targets EXOSC6 in WB, IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, FC (Intra), Indirect ELISA Application Description
经测试反应性 human
免疫原 EXOSC6 fusion protein Ag33513 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 exosome component 6
别名 241535C3, EAP4, exosome component 6, hMtr3, hMtr3p
计算分子量28kd
观测分子量28-32 kDa
GenBank蛋白编号NM_058219
基因名称 EXOSC6
Gene ID (NCBI) 118460
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDQ5RKV6
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

EXOSC6 (exosome component 6), also known as p11 or MTR3. The calculated molecular weight of EXOSC6 is 28 kDa. And it has low tissue specificity. The gene product constitutes one of the subunits of the multisubunit particle called exosome, which mediates mRNA degradation. It is also a component of the RNA exosome complex (PMID: 29906447). The composition of human exosome is similar to its yeast counterpart. EXOSC6 is homologous to the yeast Mtr3 protein. Its exact function is not known, however, it has been shown using a cell-free RNA decay system that the exosome is required for rapid degradation of unstable mRNAs containing AU-rich elements (AREs), but not for poly(A) shortening. The exosome does not recognize ARE-containing mRNAs on its own, but requires ARE-binding proteins that could interact with the exosome and recruit it to unstable mRNAs, thereby promoting their rapid degradation.