验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells, HepG2 cells, Jurkat cells, K-562 cells, MCF-7 cells, HaCat cells, HEK-293 cells |
| Positive IF/ICC detected in | HeLa cells |
| Positive FC (Intra) detected in | HeLa cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
84346-4-RR targets EXOSC6 in WB, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, IF/ICC, FC (Intra), ELISA Application Description |
| 经测试反应性 | human |
| 免疫原 | EXOSC6 fusion protein Ag33513 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | exosome component 6 |
| 别名 | 241535C3, EAP4, exosome component 6, hMtr3, hMtr3p |
| 计算分子量 | 28kd |
| 观测分子量 | 28-32 kDa |
| GenBank蛋白编号 | NM_058219 |
| 基因名称 | EXOSC6 |
| Gene ID (NCBI) | 118460 |
| RRID | AB_3671887 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purfication |
| UNIPROT ID | Q5RKV6 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
EXOSC6 (exosome component 6), also known as p11 or MTR3. The calculated molecular weight of EXOSC6 is 28 kDa. And it has low tissue specificity. The gene product constitutes one of the subunits of the multisubunit particle called exosome, which mediates mRNA degradation. It is also a component of the RNA exosome complex (PMID: 29906447). The composition of human exosome is similar to its yeast counterpart. EXOSC6 is homologous to the yeast Mtr3 protein. Its exact function is not known, however, it has been shown using a cell-free RNA decay system that the exosome is required for rapid degradation of unstable mRNAs containing AU-rich elements (AREs), but not for poly(A) shortening. The exosome does not recognize ARE-containing mRNAs on its own, but requires ARE-binding proteins that could interact with the exosome and recruit it to unstable mRNAs, thereby promoting their rapid degradation.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for EXOSC6 antibody 84346-4-RR | Download protocol |
| IF protocol for EXOSC6 antibody 84346-4-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |