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  • Featured Product
  • KD/KO Validated

CoraLite® Plus 488-conjugated EYA1 Polyclonal antibody

EYA1 Polyclonal Antibody for FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat no : CL488-22658

Print datasheet

Synonyms

EC:3.1.3.16, BOR, BOP

验证数据展示

  • FC experiment of HeLa using CL488-22658

    FC experiment of HeLa using CL488-22658

    1x10^6 HeLa cells were intracellularly stained with 0.8 ug Coralite® Plus 488 Eya1 Polyclonal Antibody (CL488-22658)(red), or 0.8 ug CoraLite® Plus 488-conjugated Rabbit IgG control Rabbit PolyAb (CL488-30000) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

经过测试的应用

Positive FC (Intra) detected inHeLa cells
For other applications, we recommend the unconjugated version of this antibody, 22658-1-AP

推荐稀释比

ApplicationDilution
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-22658 targets EYA1 in FC (Intra) applications and shows reactivity with human, mouse, rat samples.

Tested Applications FC (Intra) Application Description
Tested Reactivity human, mouse, rat
Immunogen EYA1 fusion protein Ag18489 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name eyes absent homolog 1 (Drosophila)
Synonyms EC:3.1.3.16, BOR, BOP
Calculated Molecular Weight 592 aa, 65 kDa
Observed Molecular Weight 60 kDa, 50 kDa
GenBank Accession NumberBC121799
Gene Symbol EYA1
Gene ID (NCBI) 2138
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ99502
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The EYA1 (Eyes absent homolog 1) gene is the human homologue of the Drosophila Eya1 gene, which is essential for eye development in that species. There are 4 different isoforms of EYA1 (EYA1A, EYA1B, EYA1C, EYA1D), which are composed of 559 amino acids (AA), 592 AA, 592 AA and 557 AA, respectively. It should be noted that isoforms B and C have the same length; however, they could be considered different variants because of change in amino acidic composition (PMID: 24803398).

实验方案

Product Specific Protocols
FC protocol for CL Plus 488 EYA1 antibody CL488-22658Download protocol
Standard Protocols
Click here to view our Standard Protocols