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验证数据展示

  • IF Staining of HeLa using CL488-22658

    IF Staining of HeLa using CL488-22658

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CoraLite® Plus 488 EYA1 antibody (CL488-22658) at dilution of 1:200.

  • FC experiment of HeLa using CL488-22658

    FC experiment of HeLa using CL488-22658

    1x10^6 HeLa cells were intracellularly stained with 0.8 ug Coralite® Plus 488 Eya1 Polyclonal Antibody (CL488-22658)(red), or 0.8 ug CoraLite® Plus 488-conjugated Rabbit IgG control Rabbit PolyAb (CL488-30000) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

  • Featured Product
  • KD/KO验证

CoraLite® Plus 488-conjugated EYA1 Polyclonal antibody

EYA1 Polyclonal Antibody for IF/ICC, FC (Intra)
Cat No. CL488-22658

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

IF/ICC, FC (Intra)

别名

EC:3.1.3.16, BOR, BOP

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive IF/ICC detected inHeLa cells
Positive FC (Intra) detected inHeLa cells
For other applications, we recommend the unconjugated version of this antibody, 22658-1-AP

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-22658 targets EYA1 in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

经测试应用 IF/ICC, FC (Intra) Application Description
经测试反应性 human, mouse, rat
免疫原 EYA1 fusion protein Ag18489 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 eyes absent homolog 1 (Drosophila)
别名 EC:3.1.3.16, BOR, BOP
计算分子量 592 aa, 65 kDa
观测分子量 60 kDa, 50 kDa
GenBank蛋白编号BC121799
基因名称 EYA1
Gene ID (NCBI) 2138
RRIDAB_3672747
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ99502
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The EYA1 (Eyes absent homolog 1) gene is the human homologue of the Drosophila Eya1 gene, which is essential for eye development in that species. There are 4 different isoforms of EYA1 (EYA1A, EYA1B, EYA1C, EYA1D), which are composed of 559 amino acids (AA), 592 AA, 592 AA and 557 AA, respectively. It should be noted that isoforms B and C have the same length; however, they could be considered different variants because of change in amino acidic composition (PMID: 24803398).

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 EYA1 antibody CL488-22658Download protocol
FC protocol for CL Plus 488 EYA1 antibody CL488-22658Download protocol
Standard Protocols
Click here to view our Standard Protocols