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Anti-Human Endoglin/CD105 Rabbit Recombinant Antibody, PBS Only

Endoglin/CD105 Uni-rAbTM Recombinant Antibody for FC

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC

Conjugate

Unconjugated

CloneNo.

230047E5

Cat no : 98013-1-PBS

Print datasheet

Synonyms

CD105, 230047E5, HHT1, ENG, Endoglin

验证数据展示

  • FC experiment of human PBMCs using 98013-1-PBS

    FC experiment of human PBMCs using 98013-1-PBS

    1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human Endoglin/CD105 (98013-1-PBS, Clone:230047E5) or 0.25 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) and PE-conjugated Goat Anti-Rabbit IgG. Cells were not fixed.

  • FC experiment of human PBMCs using 98013-1-RR (same clone as 98013-1-PBS)

    FC experiment of human PBMCs using 98013-1-RR (same clone as 98013-1-PBS)

    1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human Endoglin/CD105 Rabbit Recombinant Antibody (98013-1-RR, Clone:230047E5) and PE-conjugated Goat Anti-Rabbit IgG(red), or 0.25 ug Isotype Control (blue). Cells were not fixed. Monocytes were gated. This data was developed using the same antibody clone with 98013-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 98013-1-RR

    Affinity and Kinetic Characterization of 98013-1-RR

    Biolayer interferometry (BLl) kinetic assays of 98013-1-RR against Human Endoglin/CD105 were performed. The affinity constant is 6.83 nM.

经过测试的应用

Positive FC detected inhuman PBMCs
Planning an IHC experiment? We recommend our IHCeasy CD105 Ready-To-Use IHC Kit. CD105 primary antibody included.

推荐稀释比

ApplicationDilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

98013-1-PBS targets Endoglin/CD105 in FC applications and shows reactivity with human samples.

Tested Applications FC Application Description
Tested Reactivity human
Immunogen Recombinant protein 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name endoglin
Synonyms CD105, 230047E5, HHT1, ENG, Endoglin
Calculated Molecular Weight 71 kDa
GenBank Accession NumberBC014271
Gene Symbol CD105
Gene ID (NCBI) 2022
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP17813
Storage Buffer PBS only
Storage ConditionsStore at -80°C. Stable for one year after shipment.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Endoglin (ENG, CD105) is a homodimeric cell membrane glycoprotein of 180 kDa, composed of disulphide-linked subunits of 90-95 kDa. Endoglin is a proliferation-associated and hypoxia-inducible protein mainly expressed on vascular endothelial cells. It acts as an accessory receptor for transforming growth factor beta (TFG-β) and is involved in vascular development and remodeling. The important role of Endoglin in angiogenesis and in tumor progression makes it an ideal target for antiangiogenic therapy and a good marker for tumor prognosis. (PMID: 1326540; 14528280; 21737653; 12773481)

实验方案

Product Specific Protocols
FC protocol for Endoglin/CD105 antibody 98013-1-PBSDownload protocol
Standard Protocols
Click here to view our Standard Protocols