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验证数据展示

  • FC experiment of Balb/c mouse peritoneal macrophages using 98236-1-RR (same clone as 98236-1-PBS)

    FC experiment of Balb/c mouse peritoneal macrophages using 98236-1-RR (same clone as 98236-1-PBS)

    1x10^6 Balb/c mouse peritoneal macrophages were surface stained with 0.25 ug Anti-Mouse F4/80 Rabbit Recombinant Antibody (98236-1-RR, Clone: 241959G4) or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were then stained with CoraLite® Plus 647 Anti-Mouse CD11b. Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed. This data was developed using the same antibody clone with 98236-1-PBS in a different storage buffer formulation.

  • FC experiment of Balb/c mouse peritoneal macrophages using 98236-1-RR (same clone as 98236-1-PBS)

    FC experiment of Balb/c mouse peritoneal macrophages using 98236-1-RR (same clone as 98236-1-PBS)

    1x10^6 Balb/c mouse peritoneal macrophages were surface stained with 0.25 ug Anti-Mouse F4/80 Rabbit Recombinant Antibody (98236-1-RR, Clone: 241959G4) (red) or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed. This data was developed using the same antibody clone with 98236-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 98236-1-RR

    Affinity and Kinetic Characterization of 98236-1-RR

    Biolayer interferometry (BLl) kinetic assays of 98236-1-RR against Mouse F4/80 were performed. The affinity constant is 4.50 nM.

Anti-Mouse F4/80 Rabbit Recombinant Antibody, PBS Only

F4/80 Uni-rAbTM Recombinant Antibody for FC
Cat No. 98236-1-PBS

产品说明书

CloneNo. 241959G4

宿主/亚型

Rabbit / IgG

种属反应性

mouse

应用

FC

别名

Emr1, EGF-like module-containing mucin-like hormone receptor-like 1, EGF-like module receptor 1, Adhesion G protein-coupled receptor E1, Adgre1

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy Mouse F4/80 Ready-To-Use IHC Kit. Mouse F4/80 primary antibody included.

产品信息

98236-1-PBS targets F4/80 in FC applications and shows reactivity with mouse samples.

经测试应用 FC Application Description
经测试反应性 mouse
免疫原 Recombinant protein 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 EGF-like module containing, mucin-like, hormone receptor-like sequence 1
别名 Emr1, EGF-like module-containing mucin-like hormone receptor-like 1, EGF-like module receptor 1, Adhesion G protein-coupled receptor E1, Adgre1
计算分子量102 kDa
GenBank蛋白编号NM_010130.4
基因名称 F4/80
Gene ID (NCBI) 13733
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDQ61549
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Mouse F4/80, also named as EMR1 and Gpf480, is a 160-kDa cell surface glycoprotein which is a member of the EGF TM7 family. The F4/80 molecule is solely expressed on the surface of macrophages and serves as a marker for mature macrophage tissues, including Kupffer cells in liver, splenic red pulp macrophages, brain microglia, gut lamina propria and Langerhans cells in the skin. The function of F4/80 is unclear, but it is speculated to be involved in macrophage adhesion events, cell migration or as a G protein-coupled signaling component of macrophages.