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验证数据展示

  • FC experiment of Balb/c mouse peritoneal macrophages using 98236-1-RR

    FC experiment of Balb/c mouse peritoneal macrophages using 98236-1-RR

    1x10^6 Balb/c mouse peritoneal macrophages were surface stained with 0.25 ug Anti-Mouse F4/80 Rabbit RecAb (98236-1-RR, Clone: 241959G4) or Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were then stained with APC Anti-Mouse CD11b (M1/70) (APC-65055, Clone: M1/70). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.

  • FC experiment of Balb/c mouse peritoneal macrophages using 98236-1-RR

    FC experiment of Balb/c mouse peritoneal macrophages using 98236-1-RR

    1x10^6 Balb/c mouse peritoneal macrophages were surface stained with 0.25 ug Anti-Mouse F4/80 Rabbit RecAb (98236-1-RR, Clone: 241959G4) (red) or Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.

  • Affinity and Kinetic Characterization of 98236-1-RR

    Affinity and Kinetic Characterization of 98236-1-RR

    Biolayer interferometry (BLl) kinetic assays of 98236-1-RR against Mouse F4/80 were performed. The affinity constant is 4.50 nM.

Anti-Mouse F4/80 Rabbit Recombinant Antibody

F4/80 Uni-rAb® Recombinant Antibody for FC
Cat No. 98236-1-RR

产品说明书

CloneNo. 241959G4
引用文献(2)

宿主/亚型

Rabbit / IgG

种属反应性

mouse

应用

FC

别名

241959G4, Adgre1, Adhesion G protein-coupled receptor E1, EGF-like module receptor 1, EGF-like module-containing mucin-like hormone receptor-like 1

缓冲液配方:  PBS, Azide
PBS, Azide
PBS Only
偶联物:  Unconjugated
规格价格库存

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经过测试的应用

Positive FC detected inBalb/c mouse peritoneal macrophages
Planning an IHC experiment? We recommend our IHCeasy Mouse F4/80 Ready-To-Use IHC Kit. Mouse F4/80 primary antibody included.

推荐稀释比

应用推荐稀释比
Flow Cytometry (FC)FC : 0.25 ug per 10^6 cells in 100 μl suspension
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

FCSee 1 publications below

产品信息

98236-1-RR targets F4/80 in FC applications and shows reactivity with mouse samples.

经测试应用 FC Application Description
文献引用应用FC
经测试反应性 mouse
文献引用反应性mouse
免疫原

CatNo: Eg0788

Product name: Recombinant Mouse EMR1/ADGRE1/(F4/80) protein (His Tag)

Source: mammalian cells-derived, pHZ-KIsec-C-6*HIS

Tag: C-6*HIS

Domain: 28-644 aa of NM_010130.4

Sequence: QTLGGVNECQDTTTCPAYATCTDTTDSYYCTCKRGFLSSNGQTNFQGPGVECQDVNECLQSDSPCGPNSVCTNILGRAKCSCLRGFSSSTGKDWILGSLDNFLCADVDECLTIGICPKYSNCSNSVGSYSCTCQPGFVLNGSICEDEDECVTRDVCPEHATCHNTLGSYYCTCNSGLESSGGGPMFQGLDESCEDVDECSRNSTLCGPTFICINTLGSYSCSCPAGFSLPTFQILGHPADGNCTDIDECDDTCPLNSSCTNTIGSYFCTCHPGFASSNGQLNFKDLEVTCEDIDECTQDPLQCGLNSVCTNVPGSYICGCLPDFQMDPEGSQGYGNFNCKRILFKCKEDLILQSEQIQQCQAVQGRDLGYASFCTLVNATFTILDNTCENKSAPVSLQSAATSVSLVLEQATTWFELSKEETSTLGTILLETVESTMLAALLIPSGNASQMIQTEYLDIESKVINEECKENESINLAARGDKMNVGCFIIKESVSTGAPGVAFVSFAHMESVLNERFFEDGQSFRKLRMNSRVVGGTVTGEKKEDFSKPIIYTLQHIQPKQKSERPICVSWNTDVEDGRWTPSGCEIVEASETHTVCSCNRMANLAIIMASGELTME

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 EGF-like module containing, mucin-like, hormone receptor-like sequence 1
别名 241959G4, Adgre1, Adhesion G protein-coupled receptor E1, EGF-like module receptor 1, EGF-like module-containing mucin-like hormone receptor-like 1
计算分子量102 kDa
GenBank蛋白编号NM_010130.4
基因名称 F4/80
Gene ID (NCBI) 13733
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purfication
UNIPROT IDQ61549
储存缓冲液 PBS with 0.09% sodium azide, pH 7.3.
储存条件Store at 2 - 8°C. Stable for one year after shipment.

背景介绍

Mouse F4/80, also named as EMR1 and Gpf480, is a 160-kDa cell surface glycoprotein which is a member of the EGF TM7 family. The F4/80 molecule is solely expressed on the surface of macrophages and serves as a marker for mature macrophage tissues, including Kupffer cells in liver, splenic red pulp macrophages, brain microglia, gut lamina propria and Langerhans cells in the skin. The function of F4/80 is unclear, but it is speculated to be involved in macrophage adhesion events, cell migration or as a G protein-coupled signaling component of macrophages.

实验方案

Product Specific Protocols
FC protocol for F4/80 antibody 98236-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseFC

Inflammation

Moxibustion Alleviates Inflammation via SIRT5-mediated Post-translational Modification and Macrophage Polarization

Authors - Chuan-Yi Zuo
View Article

Can J Gastroenterol Hepatol

Kupffer-Cell-Targeted Carboxylesterase 1f Knockdown Deteriorates Lipopolysaccharide/D-Galactosamine-Induced Acute Liver Failure Through Regulating Cellular Polarization in Mice

Authors - Sai Zhao
View Article
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