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验证数据展示

  • FC experiment of mouse peritoneal macrophages using CL405-98236

    FC experiment of mouse peritoneal macrophages using CL405-98236

    1x10^6 mouse peritoneal macrophages were surface stained with APC Anti-Mouse CD11b, and 0.25 ug CoraLite® Plus 405 Anti-Mouse F4/80 Rabbit RecAb (CL405-98236, Clone:241959G4) or 0.25 ug CoraLite® Plus 405 Rabbit IgG Isotype Control RecAb (CL405-98136, Clone: 240953C9). Cells were incubated with FC Receptor Block prior to fixing and staining. Cells were not fixed.

  • FC experiment of mouse peritoneal macrophages using CL405-98236

    FC experiment of mouse peritoneal macrophages using CL405-98236

    1x10^6 mouse peritoneal macrophages were surface stained with 0.25 ug CoraLite® Plus 405 Anti-Mouse F4/80 Rabbit RecAb (CL405-98236, Clone: 241959G4) (red) or 0.25 ug CoraLite® Plus 405 Rabbit IgG Isotype Control RecAb (CL405-98136, Clone: 240953C9) (blue). Cells were incubated with FC Receptor Block prior to fixing and staining. Cells were not fixed.

CoraLite® Plus 405 Anti-Mouse F4/80 Rabbit Recombinant Antibody

F4/80 Uni-rAbTM Recombinant Antibody for FC
Cat No. CL405-98236

CloneNo.

241959G4

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

FC

Synonyms

Emr1, EGF-like module-containing mucin-like hormone receptor-like 1, EGF-like module receptor 1, Adhesion G protein-coupled receptor E1, Adgre1

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite® Plus 405
Size: 

-/ -

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经过测试的应用

Positive FC detected inmouse peritoneal macrophages
Planning an IHC experiment? We recommend our IHCeasy Mouse F4/80 Ready-To-Use IHC Kit. Mouse F4/80 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 98236-1-PBS

推荐稀释比

ApplicationDilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL405-98236 targets F4/80 in FC applications and shows reactivity with mouse samples.

Tested Applications FC Application Description
Tested Reactivity mouse
Immunogen Recombinant protein 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name EGF-like module containing, mucin-like, hormone receptor-like sequence 1
Synonyms Emr1, EGF-like module-containing mucin-like hormone receptor-like 1, EGF-like module receptor 1, Adhesion G protein-coupled receptor E1, Adgre1
Calculated Molecular Weight102 kDa
GenBank Accession NumberNM_010130.4
Gene Symbol F4/80
Gene ID (NCBI) 13733
Conjugate CoraLite® Plus 405 Fluorescent Dye
Excitation/Emission Maxima Wavelengths399 nm / 422 nm
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ61549
Storage Buffer PBS with 0.09% sodium azide.
Storage ConditionsStore at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

背景介绍

Mouse F4/80, also named as EMR1 and Gpf480, is a 160-kDa cell surface glycoprotein which is a member of the EGF TM7 family. The F4/80 molecule is solely expressed on the surface of macrophages and serves as a marker for mature macrophage tissues, including Kupffer cells in liver, splenic red pulp macrophages, brain microglia, gut lamina propria and Langerhans cells in the skin. The function of F4/80 is unclear, but it is speculated to be involved in macrophage adhesion events, cell migration or as a G protein-coupled signaling component of macrophages.

实验方案

Product Specific Protocols
FC protocol for CL Plus 405 F4/80 antibody CL405-98236Download protocol
Standard Protocols
Click here to view our Standard Protocols