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验证数据展示

  • FC experiment of mouse peritoneal macrophages using Biotin-98236

    FC experiment of mouse peritoneal macrophages using Biotin-98236

    1x10^6 mouse peritoneal macrophages were surface stained with 0.25 ug Biotin Anti-Mouse F4/80 Rabbit RecAb (Biotin-98236, Clone: 241959G4) and CoraLite®488-conjugated streptavidin, or unstained. Cells were co-stained with APC Anti-Mouse CD11b (M1/70) (APC-65055, Clone: M1/70). Cells were incubated with FC Receptor Block prior to fixing and staining. Cells were not fixed.

  • FC experiment of mouse peritoneal macrophages using Biotin-98236

    FC experiment of mouse peritoneal macrophages using Biotin-98236

    1x10^6 mouse peritoneal macrophages were surface stained with 0.25 ug Biotin Anti-Mouse F4/80 Rabbit RecAb (Biotin-98236, Clone: 241959G4) and CoraLite®488-conjugated streptavidin (red), or unstained (blue). Cells were incubated with FC Receptor Block prior to fixing and staining. Cells were not fixed.

Biotin Anti-Mouse F4/80 Rabbit Recombinant Antibody

F4/80 Uni-rAb® Recombinant Antibody for FC
Cat No. Biotin-98236

产品说明书

CloneNo. 241959G4

宿主/亚型

Rabbit / IgG

种属反应性

mouse

应用

FC

别名

Emr1, EGF-like module-containing mucin-like hormone receptor-like 1, EGF-like module receptor 1, Adhesion G protein-coupled receptor E1, Adgre1

缓冲液配方:  PBS, Azide
PBS, Azide
偶联物:  Biotin
规格价格库存

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经过测试的应用

Positive FC detected inmouse peritoneal macrophages
Planning an IHC experiment? We recommend our IHCeasy Mouse F4/80 Ready-To-Use IHC Kit. Mouse F4/80 primary antibody included.

推荐稀释比

应用推荐稀释比
Flow Cytometry (FC)FC : 0.25 ug per 10^6 cells in 100 μl suspension
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

Biotin-98236 targets F4/80 in FC applications and shows reactivity with mouse samples.

经测试应用 FC Application Description
经测试反应性 mouse
免疫原

Recombinant protein

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 EGF-like module containing, mucin-like, hormone receptor-like sequence 1
别名 Emr1, EGF-like module-containing mucin-like hormone receptor-like 1, EGF-like module receptor 1, Adhesion G protein-coupled receptor E1, Adgre1
计算分子量102 kDa
GenBank蛋白编号NM_010130.4
基因名称 F4/80
Gene ID (NCBI) 13733
偶联类型 Biotin
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ61549
储存缓冲液 PBS with 0.09% sodium azide, pH 7.3.
储存条件Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

背景介绍

Mouse F4/80, also named as EMR1 and Gpf480, is a 160-kDa cell surface glycoprotein which is a member of the EGF TM7 family. The F4/80 molecule is solely expressed on the surface of macrophages and serves as a marker for mature macrophage tissues, including Kupffer cells in liver, splenic red pulp macrophages, brain microglia, gut lamina propria and Langerhans cells in the skin. The function of F4/80 is unclear, but it is speculated to be involved in macrophage adhesion events, cell migration or as a G protein-coupled signaling component of macrophages.

实验方案

Product Specific Protocols
FC protocol for Biotin F4/80 antibody Biotin-98236Download protocol
Standard Protocols
Click here to view our Standard Protocols
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