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验证数据展示

  • FC experiment of mouse peritoneal macrophages using Biotin-98236

    FC experiment of mouse peritoneal macrophages using Biotin-98236

    1x10^6 mouse peritoneal macrophages were surface stained with 0.25 ug Biotin Anti-Mouse F4/80 Rabbit RecAb (Biotin-98236, Clone: 241959G4) and CoraLite®488-conjugated streptavidin, or unstained. Cells were co-stained with APC Anti-Mouse CD11b (M1/70) (APC-65055, Clone: M1/70). Cells were incubated with FC Receptor Block prior to fixing and staining. Cells were not fixed.

  • FC experiment of mouse peritoneal macrophages using Biotin-98236

    FC experiment of mouse peritoneal macrophages using Biotin-98236

    1x10^6 mouse peritoneal macrophages were surface stained with 0.25 ug Biotin Anti-Mouse F4/80 Rabbit RecAb (Biotin-98236, Clone: 241959G4) and CoraLite®488-conjugated streptavidin (red), or unstained (blue). Cells were incubated with FC Receptor Block prior to fixing and staining. Cells were not fixed.

Biotin Anti-Mouse F4/80 Rabbit Recombinant Antibody

F4/80 Uni-rAbTM Recombinant Antibody for FC
Cat No. Biotin-98236

Print datasheet

CloneNo. 241959G4

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

FC

Synonyms

Emr1, EGF-like module-containing mucin-like hormone receptor-like 1, EGF-like module receptor 1, Adhesion G protein-coupled receptor E1, Adgre1

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Biotin
Size: 

-/ -

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经过测试的应用

Positive FC detected inmouse peritoneal macrophages
Planning an IHC experiment? We recommend our IHCeasy Mouse F4/80 Ready-To-Use IHC Kit. Mouse F4/80 primary antibody included.

推荐稀释比

ApplicationDilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

Biotin-98236 targets F4/80 in FC applications and shows reactivity with mouse samples.

Tested Applications FC Application Description
Tested Reactivity mouse
Immunogen Recombinant protein 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name EGF-like module containing, mucin-like, hormone receptor-like sequence 1
Synonyms Emr1, EGF-like module-containing mucin-like hormone receptor-like 1, EGF-like module receptor 1, Adhesion G protein-coupled receptor E1, Adgre1
Calculated Molecular Weight102 kDa
GenBank Accession NumberNM_010130.4
Gene Symbol F4/80
Gene ID (NCBI) 13733
Conjugate Biotin
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ61549
Storage Buffer PBS with 0.09% sodium azide.
Storage ConditionsStore at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

背景介绍

Mouse F4/80, also named as EMR1 and Gpf480, is a 160-kDa cell surface glycoprotein which is a member of the EGF TM7 family. The F4/80 molecule is solely expressed on the surface of macrophages and serves as a marker for mature macrophage tissues, including Kupffer cells in liver, splenic red pulp macrophages, brain microglia, gut lamina propria and Langerhans cells in the skin. The function of F4/80 is unclear, but it is speculated to be involved in macrophage adhesion events, cell migration or as a G protein-coupled signaling component of macrophages.

实验方案

Product Specific Protocols
FC protocol for Biotin F4/80 antibody Biotin-98236Download protocol
Standard Protocols
Click here to view our Standard Protocols