验证数据展示
经过测试的应用
Positive WB detected in | LNCaP cells, pig liver tissue, rat liver tissue, mouse liver tissue, HepG2 cells, Jurkat cells, K-562 cells |
Positive IF/ICC detected in | HepG2 cells |
推荐稀释比
Application | Dilution |
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Western Blot (WB) | WB : 1:5000-1:50000 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
67986-1-Ig targets FAH in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat, pig samples.
Tested Applications | WB, IF/ICC, ELISA Application Description |
Tested Reactivity | human, mouse, rat, pig |
Immunogen | FAH fusion protein Ag6704 种属同源性预测 |
Host / Isotype | Mouse / IgG1 |
Class | Monoclonal |
Type | Antibody |
Full Name | fumarylacetoacetate hydrolase (fumarylacetoacetase) |
Synonyms | Fumarylacetoacetate hydrolase, FAA, EC:3.7.1.2, Beta-diketonase, 2F8E11 |
Calculated Molecular Weight | 46 kDa |
Observed Molecular Weight | 40-45 kDa |
GenBank Accession Number | BC002527 |
Gene Symbol | FAH |
Gene ID (NCBI) | 2184 |
RRID | AB_2918735 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purification |
UNIPROT ID | P16930 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
FAH is also named as FAA(fumarylacetoacetase), the last enzyme in tyrosine degradation, functionally important in the liver and kidney(PMID:8473520). FAH is a metabolic enzyme catalyzing the last step of tyrosine and phenylalanine catabolism: the hydrolysis of fumarylacetoacetate into acetoacetate and fumarate. In humans, deficiency of this activity is associated with the metabolic disease hereditary tyrosinaemia type I, which is also known as hepatorenal tyrosinaemia(PMID:17768357).
实验方案
Product Specific Protocols | |
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WB protocol for FAH antibody 67986-1-Ig | Download protocol |
IF protocol for FAH antibody 67986-1-Ig | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |