验证数据展示
经过测试的应用
| Positive IF/ICC detected in | HepG2 cells |
For other applications, we recommend the unconjugated version of this antibody, 67986-1-Ig
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL555-67986 targets FAH in IF/ICC applications and shows reactivity with Human, pig, rat, mouse samples.
| 经测试应用 | IF/ICC Application Description |
| 经测试反应性 | Human, pig, rat, mouse |
| 免疫原 | FAH fusion protein Ag6704 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG1 |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | fumarylacetoacetate hydrolase (fumarylacetoacetase) |
| 别名 | Beta diketonase, FAA, FAH, Fumarylacetoacetase, Fumarylacetoacetate hydrolase |
| 计算分子量 | 46 kDa |
| 观测分子量 | 40-45 kDa |
| GenBank蛋白编号 | BC002527 |
| 基因名称 | FAH |
| Gene ID (NCBI) | 2184 |
| RRID | AB_2934687 |
| 偶联类型 | CoraLite®555 Fluorescent Dye |
| 最大激发/发射波长 | 557 nm / 570nm |
| 形式 | Liquid |
| 纯化方式 | Protein G purification |
| UNIPROT ID | P16930 |
| 储存缓冲液 | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| 储存条件 | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
FAH is also named as FAA(fumarylacetoacetase), the last enzyme in tyrosine degradation, functionally important in the liver and kidney(PMID:8473520). FAH is a metabolic enzyme catalyzing the last step of tyrosine and phenylalanine catabolism: the hydrolysis of fumarylacetoacetate into acetoacetate and fumarate. In humans, deficiency of this activity is associated with the metabolic disease hereditary tyrosinaemia type I, which is also known as hepatorenal tyrosinaemia(PMID:17768357).
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for CL555 FAH antibody CL555-67986 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |