验证数据展示
经过测试的应用
Positive WB detected in | fetal human brain tissue |
Positive IHC detected in | human oesophagus cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF/ICC detected in | HepG2 cells |
推荐稀释比
Application | Dilution |
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Western Blot (WB) | WB : 1:500-1:2000 |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
60282-1-Ig targets FAM3C in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.
Tested Applications | WB, IHC, IF/ICC, ELISA Application Description |
Tested Reactivity | human |
Immunogen | FAM3C fusion protein Ag5516 种属同源性预测 |
Host / Isotype | Mouse / IgG1 |
Class | Monoclonal |
Type | Antibody |
Full Name | family with sequence similarity 3, member C |
Synonyms | Protein FAM3C, Interleukin-like EMT inducer, Interleukin like EMT inducer, ILEI, GS3786 |
Calculated Molecular Weight | 25 kDa |
Observed Molecular Weight | 25 kDa |
GenBank Accession Number | BC046932 |
Gene Symbol | FAM3C |
Gene ID (NCBI) | 10447 |
RRID | AB_2881400 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purification |
UNIPROT ID | Q92520 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
FAM3C, also known as ILEI, is a secreted protein belonging to the cytokine-like protein family. FAM3C is a signaling cytokine with a GG domain, hydrophobic leader sequence and an N-terminal signal peptide. A change in expression of this protein has been noted in pancreatic cancer-derived cells. FAM3C has been involved in the epithelial to mesenchymal transition, and retinal laminar formation processes in vertebrates. Also, FAM3C may play an important role in inner ear development and thus be involved in cell differentiation and proliferation during inner ear embryogenesis.
实验方案
Product Specific Protocols | |
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WB protocol for FAM3C antibody 60282-1-Ig | Download protocol |
IHC protocol for FAM3C antibody 60282-1-Ig | Download protocol |
IF protocol for FAM3C antibody 60282-1-Ig | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |