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验证数据展示

  • IF Staining of HepG2 using CL594-60282

    IF Staining of HepG2 using CL594-60282

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CL594-60282 (FAM3C antibody) at dilution of 1:100.

CoraLite®594-conjugated FAM3C Monoclonal antibody

FAM3C Monoclonal Antibody for IF/ICC
Cat No. CL594-60282

产品说明书

CloneNo. 2E10D5

宿主/亚型

Mouse / IgG1

种属反应性

human

应用

IF/ICC

别名

FAM3C, GS3786, ILEI, Interleukin like EMT inducer, Protein FAM3C

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite®594
规格: 

-/ -

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经过测试的应用

Positive IF/ICC detected inHepG2 cells
For other applications, we recommend the unconjugated version of this antibody, 60282-1-Ig

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL594-60282 targets FAM3C in IF/ICC applications and shows reactivity with human samples.

经测试应用 IF/ICC Application Description
经测试反应性 human
免疫原 FAM3C fusion protein Ag5516 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 family with sequence similarity 3, member C
别名 FAM3C, GS3786, ILEI, Interleukin like EMT inducer, Protein FAM3C
计算分子量 25 kDa
观测分子量 25 kDa
GenBank蛋白编号BC046932
基因名称 FAM3C
Gene ID (NCBI) 10447
RRIDAB_2883453
偶联类型 CoraLite®594 Fluorescent Dye
最大激发/发射波长588 nm / 604 nm
形式 Liquid
纯化方式Protein G purification
UNIPROT IDQ92520
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

FAM3C, also known as ILEI, is a secreted protein belonging to the cytokine-like protein family. FAM3C is a signaling cytokine with a GG domain, hydrophobic leader sequence and an N-terminal signal peptide. A change in expression of this protein has been noted in pancreatic cancer-derived cells. FAM3C has been involved in the epithelial to mesenchymal transition, and retinal laminar formation processes in vertebrates. Also, FAM3C may play an important role in inner ear development and thus be involved in cell differentiation and proliferation during inner ear embryogenesis.

实验方案

Product Specific Protocols
IF protocol for CL594 FAM3C antibody CL594-60282Download protocol
Standard Protocols
Click here to view our Standard Protocols