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验证数据展示

  • WB analysis of fetal human brain using 60282-1-Ig (same clone as 60282-1-PBS)

    WB analysis of fetal human brain using 60282-1-Ig (same clone as 60282-1-PBS)

    fetal human brain tissue were subjected to SDS PAGE followed by western blot with 60282-1-Ig (FAM3C Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60282-1-PBS in a different storage buffer formulation.

  • IHC staining of human oesophagus cancer using 60282-1-Ig (same clone as 60282-1-PBS)

    IHC staining of human oesophagus cancer using 60282-1-Ig (same clone as 60282-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using 60282-1-Ig (FAM3C Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60282-1-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 60282-1-Ig (same clone as 60282-1-PBS)

    IF Staining of HepG2 using 60282-1-Ig (same clone as 60282-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using FAM3C antibody (60282-1-Ig, Clone: 2E10D5 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 60282-1-PBS in a different storage buffer formulation.

  • IHC staining of human oesophagus cancer using 60282-1-Ig (same clone as 60282-1-PBS)

    IHC staining of human oesophagus cancer using 60282-1-Ig (same clone as 60282-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using 60282-1-Ig (FAM3C Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60282-1-PBS in a different storage buffer formulation.

FAM3C Monoclonal antibody, PBS Only

FAM3C Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 60282-1-PBS

产品说明书

CloneNo. 2E10D5

宿主/亚型

Mouse / IgG1

种属反应性

human

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

Protein FAM3C, Interleukin-like EMT inducer, Interleukin like EMT inducer, ILEI, GS3786

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

60282-1-PBS targets FAM3C in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 human
免疫原 FAM3C fusion protein Ag5516 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 family with sequence similarity 3, member C
别名 Protein FAM3C, Interleukin-like EMT inducer, Interleukin like EMT inducer, ILEI, GS3786
计算分子量 25 kDa
观测分子量 25 kDa
GenBank蛋白编号BC046932
基因名称 FAM3C
Gene ID (NCBI) 10447
RRIDAB_2881400
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ92520
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

FAM3C, also known as ILEI, is a secreted protein belonging to the cytokine-like protein family. FAM3C is a signaling cytokine with a GG domain, hydrophobic leader sequence and an N-terminal signal peptide. A change in expression of this protein has been noted in pancreatic cancer-derived cells. FAM3C has been involved in the epithelial to mesenchymal transition, and retinal laminar formation processes in vertebrates. Also, FAM3C may play an important role in inner ear development and thus be involved in cell differentiation and proliferation during inner ear embryogenesis.