验证数据展示
经过测试的应用
| Positive IF/ICC detected in | HepG2 cells |
Planning an IHC experiment? We recommend our IHCeasy FBL Ready-To-Use IHC Kit. FBL primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 66985-1-Ig
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL555-66985 targets FBL in IF/ICC applications and shows reactivity with Human, rat, mouse samples.
| 经测试应用 | IF/ICC Application Description |
| 经测试反应性 | Human, rat, mouse |
| 免疫原 | FBL fusion protein Ag8841 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG1 |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | fibrillarin |
| 别名 | FBL, FIB, FIB1, fibrillarin, FLRN, RNU3IP1 |
| 计算分子量 | 321 aa, 34 kDa |
| 观测分子量 | 34 kDa |
| GenBank蛋白编号 | BC019260 |
| 基因名称 | FBL |
| Gene ID (NCBI) | 2091 |
| RRID | AB_3673421 |
| 偶联类型 | CoraLite® Plus 555 Fluorescent Dye |
| 最大激发/发射波长 | 554 nm / 570 nm |
| 形式 | Liquid |
| 纯化方式 | Protein G purification |
| UNIPROT ID | P22087 |
| 储存缓冲液 | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3 |
| 储存条件 | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Fibrillarin (Fbl) is an essential nucleolar protein that plays an essential role in ribosome biogenesis and more particularly in the methylation of ribosomal RNAs and rDNA histones. FBL is highly expressed in various cancers and correlates with poor survival outcomes in cancer patients,such as hepatocellular carcinoma(PMID: 33376525). Knockdown of FBL sensitizes tumor cells and xenografts to DNA crosslinking agents, and leads to homologous recombination-mediated DNA repair defects(PMID: 37489617).
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for CL Plus 555 FBL antibody CL555-66985 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |