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验证数据展示

  • IF Staining of HepG2 using CL594-66985

    IF Staining of HepG2 using CL594-66985

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CoraLite®594 FBL antibody (CL594-66985, Clone: 3A9E8 ) at dilution of 1:200.

  • FC experiment of HepG2 using CL594-66985

    FC experiment of HepG2 using CL594-66985

    1x10^6 HepG2 cells were intracellularly stained with 0.8 ug CoraLite®594 Anti-Human FBL (CL594-66985, Clone:3A9E8)(red), or 0.8 ug CoraLite®594 Mouse IgG1 Isotype Control (MOPC-21) (CL594-65124, Clone: MOPC-21) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

CoraLite®594-conjugated FBL Monoclonal antibody

FBL Monoclonal Antibody for IF/ICC, FC (Intra)
Cat No. CL594-66985

产品说明书

CloneNo. 3A9E8

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse, rat

应用

IF/ICC, FC (Intra)

别名

FLRN, fibrillarin, FIB1, FIB, EC:2.1.1.-

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite®594
规格: 

-/ -

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经过测试的应用

Positive IF/ICC detected inHepG2 cells
Positive FC (Intra) detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy FBL Ready-To-Use IHC Kit. FBL primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 66985-1-Ig

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL594-66985 targets FBL in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

经测试应用 IF/ICC, FC (Intra) Application Description
经测试反应性 human, mouse, rat
免疫原 FBL fusion protein Ag8841 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 fibrillarin
别名 FLRN, fibrillarin, FIB1, FIB, EC:2.1.1.-
计算分子量 321 aa, 34 kDa
观测分子量 34 kDa
GenBank蛋白编号BC019260
基因名称 FBL
Gene ID (NCBI) 2091
RRIDAB_3673511
偶联类型 CoraLite®594 Fluorescent Dye
最大激发/发射波长588 nm / 604 nm
形式 Liquid
纯化方式Protein G purification
UNIPROT IDP22087
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Fibrillarin (Fbl) is an essential nucleolar protein that plays an essential role in ribosome biogenesis and more particularly in the methylation of ribosomal RNAs and rDNA histones. FBL is highly expressed in various cancers and correlates with poor survival outcomes in cancer patients,such as hepatocellular carcinoma(PMID: 33376525). Knockdown of FBL sensitizes tumor cells and xenografts to DNA crosslinking agents, and leads to homologous recombination-mediated DNA repair defects(PMID: 37489617).

实验方案

Product Specific Protocols
IF protocol for CL594 FBL antibody CL594-66985Download protocol
FC protocol for CL594 FBL antibody CL594-66985Download protocol
Standard Protocols
Click here to view our Standard Protocols