验证数据展示
经过测试的应用
Positive IF/ICC detected in | HepG2 cells |
Positive FC (Intra) detected in | HepG2 cells |
推荐稀释比
Application | Dilution |
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Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
CL594-66985 targets FBL in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, rat samples.
Tested Applications | IF/ICC, FC (Intra) Application Description |
Tested Reactivity | human, mouse, rat |
Immunogen | FBL fusion protein Ag8841 种属同源性预测 |
Host / Isotype | Mouse / IgG1 |
Class | Monoclonal |
Type | Antibody |
Full Name | fibrillarin |
Synonyms | FLRN, fibrillarin, FIB1, FIB, EC:2.1.1.- |
Calculated Molecular Weight | 321 aa, 34 kDa |
Observed Molecular Weight | 34 kDa |
GenBank Accession Number | BC019260 |
Gene Symbol | FBL |
Gene ID (NCBI) | 2091 |
RRID | AB_3673511 |
Conjugate | CoraLite®594 Fluorescent Dye |
Excitation/Emission Maxima Wavelengths | 588 nm / 604 nm |
Form | Liquid |
Purification Method | Protein G purification |
UNIPROT ID | P22087 |
Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Fibrillarin (Fbl) is an essential nucleolar protein that plays an essential role in ribosome biogenesis and more particularly in the methylation of ribosomal RNAs and rDNA histones. FBL is highly expressed in various cancers and correlates with poor survival outcomes in cancer patients,such as hepatocellular carcinoma(PMID: 33376525). Knockdown of FBL sensitizes tumor cells and xenografts to DNA crosslinking agents, and leads to homologous recombination-mediated DNA repair defects(PMID: 37489617).
实验方案
Product Specific Protocols | |
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IF protocol for CL594 FBL antibody CL594-66985 | Download protocol |
FC protocol for CL594 FBL antibody CL594-66985 | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |