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验证数据展示

  • WB analysis using CL488-67972

    WB analysis using CL488-67972

    Various lysates were subjected to SDS PAGE followed by western blot with CL488-67972 (FDPS antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • FC experiment of HeLa using CL488-67972

    FC experiment of HeLa using CL488-67972

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human FDPS (CL488-67972, Clone:3B8A7) (red), or 0.4 ug Mouse IgG2b Isotype Control (CL488-66360-3, Clone: K11B8C4B5) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

CoraLite® Plus 488-conjugated FDPS Monoclonal antibody

FDPS Monoclonal Antibody for WB, FC (Intra)
Cat No. CL488-67972

产品说明书

CloneNo. 3B8A7

宿主/亚型

Mouse / IgG2b

种属反应性

human

应用

WB, FC (Intra)

别名

Farnesyl diphosphate synthase, EC:2.5.1.10, EC:2.5.1.1, Dimethylallyltranstransferase, 3B8A7

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive WB detected inHeLa cells, Jurkat cells
Positive FC (Intra) detected inHeLa cells
For other applications, we recommend the unconjugated version of this antibody, 67972-1-Ig

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:8000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-67972 targets FDPS in WB, FC (Intra) applications and shows reactivity with human samples.

经测试应用 WB, FC (Intra) Application Description
经测试反应性 human
免疫原 FDPS fusion protein Ag9247 种属同源性预测
宿主/亚型 Mouse / IgG2b
抗体类别 Monoclonal
产品类型 Antibody
全称 farnesyl diphosphate synthase (farnesyl pyrophosphate synthetase, dimethylallyltranstransferase, geranyltranstransferase)
别名 Farnesyl diphosphate synthase, EC:2.5.1.10, EC:2.5.1.1, Dimethylallyltranstransferase, 3B8A7
计算分子量 419 aa, 48 kDa
观测分子量38-39 kDa
GenBank蛋白编号BC010004
基因名称 FDPS
Gene ID (NCBI) 2224
RRIDAB_2923844
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP14324
储存缓冲液 PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

FPPS (also known as FDPS) is a key enzyme in isoprenoid biosynthesis which catalyzes the formation of farnesyl diphosphate (FPP), a precursor for several classes of essential metabolites including sterols, dolichols, carotenoids, and ubiquinones. The expression of FPPS was elevated in prostate tumor cell lines, indicating its potential role in prostate cancer progression.

实验方案

Product Specific Protocols
WB protocol for CL Plus 488 FDPS antibody CL488-67972Download protocol
FC protocol for CL Plus 488 FDPS antibody CL488-67972Download protocol
Standard Protocols
Click here to view our Standard Protocols