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CoraLite® Plus 488-conjugated FDPS Monoclonal antibody

FDPS Monoclonal Antibody for WB, FC (Intra)

Host / Isotype

Mouse / IgG2b

Reactivity

human

Applications

WB, FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

3B8A7

Cat no : CL488-67972

Print datasheet

Synonyms

Farnesyl diphosphate synthase, EC:2.5.1.10, EC:2.5.1.1, Dimethylallyltranstransferase, 3B8A7

验证数据展示

  • WB analysis using CL488-67972

    WB analysis using CL488-67972

    Various lysates were subjected to SDS PAGE followed by western blot with CL488-67972 (FDPS antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • FC experiment of HeLa using CL488-67972

    FC experiment of HeLa using CL488-67972

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human FDPS (CL488-67972, Clone:3B8A7) (red), or 0.4 ug Mouse IgG2b Isotype Control (CL488-66360-3, Clone: K11B8C4B5) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

经过测试的应用

Positive WB detected inHeLa cells, Jurkat cells
Positive FC (Intra) detected inHeLa cells
For other applications, we recommend the unconjugated version of this antibody, 67972-1-Ig

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-67972 targets FDPS in WB, FC (Intra) applications and shows reactivity with human samples.

Tested Applications WB, FC (Intra) Application Description
Tested Reactivity human
Immunogen FDPS fusion protein Ag9247 种属同源性预测
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Full Name farnesyl diphosphate synthase (farnesyl pyrophosphate synthetase, dimethylallyltranstransferase, geranyltranstransferase)
Synonyms Farnesyl diphosphate synthase, EC:2.5.1.10, EC:2.5.1.1, Dimethylallyltranstransferase, 3B8A7
Calculated Molecular Weight 419 aa, 48 kDa
Observed Molecular Weight38-39 kDa
GenBank Accession NumberBC010004
Gene Symbol FDPS
Gene ID (NCBI) 2224
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP14324
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

FPPS (also known as FDPS) is a key enzyme in isoprenoid biosynthesis which catalyzes the formation of farnesyl diphosphate (FPP), a precursor for several classes of essential metabolites including sterols, dolichols, carotenoids, and ubiquinones. The expression of FPPS was elevated in prostate tumor cell lines, indicating its potential role in prostate cancer progression.

实验方案

Product Specific Protocols
WB protocol for CL Plus 488 FDPS antibody CL488-67972Download protocol
FC protocol for CL Plus 488 FDPS antibody CL488-67972Download protocol
Standard Protocols
Click here to view our Standard Protocols