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验证数据展示

  • WB analysis using 83352-1-RR (same clone as 83352-1-PBS)

    WB analysis using 83352-1-RR (same clone as 83352-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83352-1-RR (FGR antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83352-1-PBS in a different storage buffer formulation.

  • IF Staining of Raji using 83352-1-RR (same clone as 83352-1-PBS)

    IF Staining of Raji using 83352-1-RR (same clone as 83352-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed Raji cells using FGR antibody (83352-1-RR, Clone: 240275E5 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 83352-1-PBS in a different storage buffer formulation.

  • FC experiment of Raji using 83352-1-RR (same clone as 83352-1-PBS)

    FC experiment of Raji using 83352-1-RR (same clone as 83352-1-PBS)

    1x10^6 Raji cells were intracellularly stained with 0.25 ug FGR Recombinant antibody (83352-1-RR, Clone:240275E5) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83352-1-PBS in a different storage buffer formulation.

  • Sandwich ELISA standard curve of MP00370-1

    Sandwich ELISA standard curve of MP00370-1

    Sandwich ELISA standard curve of MP00370-1, Human FGR Recombinant Matched Antibody Pair - PBS only. 83352-1-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag34944. 83352-3-PBS was HRP conjugated as the detection antibody. Range: 0.0781-25 ng/mL

  • Sample test of MP00370-1

    Sample test of MP00370-1

    Raji cell lysate was measured. The human FGR concentration of detected samples was determined to be 23.90 ng/mL (based on a 1.2 mg/mL extract load).

  • Cytometric bead array standard curve of MP00370-1

    Cytometric bead array standard curve of MP00370-1

    Cytometric bead array standard curve of MP00370-1, FGR Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83352-1-PBS. Detection antibody: 83352-3-PBS. Standard: Ag34944. Range: 0.313-40 ng/mL.

  • Affinity and Kinetic Characterization of 83352-1-PBS

    Affinity and Kinetic Characterization of 83352-1-PBS

    Biolayer interferometry (BLl) kinetic assay of 83352-1-PBS against Human FGR was performed. The affinity constant is 28.2 pM.

  • Affinity and Kinetic Characterization of 83352-1-RR

    Affinity and Kinetic Characterization of 83352-1-RR

    Biolayer interferometry (BLl) kinetic assays of 83352-1-RR against Human FGR were performed. The affinity constant is 0.139 nM.

FGR Recombinant antibody, PBS Only (Capture)

FGR Uni-rAbTM Recombinant Antibody for WB, IF/ICC, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test
Cat No. 83352-1-PBS

产品说明书

CloneNo. 240275E5

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IF/ICC, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

别名

p58c fgr, p55c fgr, p55 Fgr, c src2, c fgr

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

83352-1-PBS targets FGR as part of a matched antibody pair:

MP00370-1: 83352-1-PBS capture and 83352-3-PBS detection (validated in Cytometric bead array, Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 WB, IF/ICC, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test Application Description
经测试反应性 human
免疫原 FGR fusion protein Ag34944 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 Gardner-Rasheed feline sarcoma viral (v-fgr) oncogene homolog
别名 p58c fgr, p55c fgr, p55 Fgr, c src2, c fgr
计算分子量59 kDa
观测分子量60 kDa
GenBank蛋白编号NM_005248
基因名称 FGR
Gene ID (NCBI) 2268
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP09769
储存缓冲液 PBS Only
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

FGR (Tyrosine-protein kinase Fgr) is a member of the Src family of protein tyrosine kinases (PTKs). It's contains N-terminal sites for myristylation and palmitylation, a PTK domain, and SH2 and SH3 domains which are involved in mediating protein-protein interactions with phosphotyrosine-containing and proline-rich motifs, respectively.