验证数据展示
经过测试的应用
| Positive IF/ICC detected in | MCF-7 cells |
Planning an IHC experiment? We recommend our IHCeasy FIP1L1 Ready-To-Use IHC Kit. FIP1L1 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 83863-3-PBS
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL488-83863-3 targets FIP1L1 in IF/ICC applications and shows reactivity with human samples.
| 经测试应用 | IF/ICC Application Description |
| 经测试反应性 | human |
| 免疫原 | FIP1L1 fusion protein Ag2921 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | FIP1 like 1 (S. cerevisiae) |
| 别名 | RHE, Pre-mRNA 3'-end-processing factor FIP1, FIP1-like 1 protein, FIP1, 240658A8 |
| 计算分子量 | 594 aa, 66 kDa |
| GenBank蛋白编号 | BC017724 |
| 基因名称 | FIP1L1 |
| Gene ID (NCBI) | 81608 |
| RRID | AB_3673317 |
| 偶联类型 | CoraLite® Plus 488 Fluorescent Dye |
| 最大激发/发射波长 | 493 nm / 522 nm |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | Q6UN15 |
| 储存缓冲液 | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3 |
| 储存条件 | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
FIP1L1, also known as FIP1, encodes a subunit of the CPSF (cleavage and polyadenylation specificity factor) complex that polyadenylates the 3' end of mRNA precursors. The FIP1L1-PDGFRA fusion gene encodes a constitutively activated tyrosine kinase that links the first 233 amino acids of FIP1L1 to the last 523 amino acids of PDGFRA. This gene fusion and chromosomal deletion causes some forms of idiopathic hypereosinophilic syndrome (HES)( PMID: 12660384).
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for CL Plus 488 FIP1L1 antibody CL488-83863-3 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |