验证数据展示
经过测试的应用
Positive WB detected in | LNCaP cells, HEK-293 cells, HeLa cells, HepG2 cells, Jurkat cells, NIH/3T3 cells, HSC-T6 cells |
Positive IP detected in | HeLa cells |
Positive IHC detected in | human prostate cancer tissue, rat brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF/ICC detected in | HepG2 cells |
Positive FC (Intra) detected in | HepG2 cells |
推荐稀释比
应用 | 推荐稀释比 |
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Western Blot (WB) | WB : 1:5000-1:50000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:500-1:2000 |
Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
82248-1-RR targets FIS1 in WB, IHC, IF/ICC, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse, rat samples.
经测试应用 | WB, IHC, IF/ICC, FC (Intra), IP, ELISA Application Description |
经测试反应性 | human, mouse, rat |
免疫原 | FIS1 fusion protein Ag1409 种属同源性预测 |
宿主/亚型 | Rabbit / IgG |
抗体类别 | Recombinant |
产品类型 | Antibody |
全称 | fission 1 (mitochondrial outer membrane) homolog (S. cerevisiae) |
别名 | TPR repeat protein 11, hFis1, FIS1 homolog, CGI-135, CGI 135 |
计算分子量 | 17 kDa |
观测分子量 | 15 kDa |
GenBank蛋白编号 | BC009428 |
基因名称 | FIS1 |
Gene ID (NCBI) | 51024 |
RRID | AB_3086472 |
偶联类型 | Unconjugated |
形式 | Liquid |
纯化方式 | Protein A purification |
UNIPROT ID | Q9Y3D6 |
储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Fis1 (fission 1) is an integral mitochondrial outer membrane protein that participates in mitochondrial fission by interacting with dynamin-related protein 1 (Drp1). Excessive mitochondrial fission is associated with the pathology of a number of neurodegenerative or neurodevelopmental diseases. Increased expression of Fis1 has been found in Huntington's disease (HD)-affected brain, Alzheimer's disease (AD) patients, and autism spectrum disorder. (PMID: 21257639, 21459773, 23333625)
实验方案
Product Specific Protocols | |
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WB protocol for FIS1 antibody 82248-1-RR | Download protocol |
IHC protocol for FIS1 antibody 82248-1-RR | Download protocol |
IF protocol for FIS1 antibody 82248-1-RR | Download protocol |
IP protocol for FIS1 antibody 82248-1-RR | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |