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CoraLite® Plus 488-conjugated FIS1 Recombinant antibody

FIS1 Recombinant Antibody for IF/ICC, FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse, rat

Applications

IF/ICC, FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

6N15

Cat No : CL488-82248

Print datasheet

Synonyms

CGI 135, FIS1, FIS1 homolog, hFis1, TPR repeat protein 11, TTC11

验证数据展示

  • FC experiment of HepG2 using CL488-82248

    FC experiment of HepG2 using CL488-82248

    1X10^6 HepG2 cells were intracellularly stained with 0.8 ug CoraLite® Plus 488 Anti-Human FIS1 (CL488-82248, Clone:6N15) (red), or 0.8 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • IF Staining of HepG2 using CL488-82248

    IF Staining of HepG2 using CL488-82248

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CoraLite® Plus 488 FIS1 antibody (CL488-82248, Clone: 6N15 ) at dilution of 1:200.

经过测试的应用

Positive IF detected inHepG2 cells
Positive FC detected inHepG2 cells
For other applications, we recommend the unconjugated version of this antibody, 82248-1-PBS

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
Flow Cytometry (FC)FC : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-82248 targets FIS1 in IF, FC (Intra) applications and shows reactivity with Human, mouse, rat samples.

Tested Applications IF/ICC, FC (Intra)
Tested Reactivity Human, mouse, rat
Immunogen FIS1 fusion protein Ag1409 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name fission 1 (mitochondrial outer membrane) homolog (S. cerevisiae)
Synonyms CGI 135, FIS1, FIS1 homolog, hFis1, TPR repeat protein 11, TTC11
Calculated Molecular Weight 17 kDa
Observed Molecular Weight15 kDa
GenBank Accession NumberBC009428
Gene Symbol FIS1
Gene ID (NCBI) 51024
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ9Y3D6
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Fis1 (fission 1) is an integral mitochondrial outer membrane protein that participates in mitochondrial fission by interacting with dynamin-related protein 1 (Drp1). Excessive mitochondrial fission is associated with the pathology of a number of neurodegenerative or neurodevelopmental diseases. Increased expression of Fis1 has been found in Huntington's disease (HD)-affected brain, Alzheimer's disease (AD) patients, and autism spectrum disorder. (PMID: 21257639, 21459773, 23333625)

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 FIS1 antibody CL488-82248Download protocol
FC protocol for CL Plus 488 FIS1 antibody CL488-82248Download protocol
Standard Protocols
Click here to view our Standard Protocols