• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
  • Featured Product
  • KD/KO Validated

FOX2/RBM9 Polyclonal antibody, PBS Only

FOX2/RBM9 Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, IP, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, IF-P, IP, Indirect ELISA

Conjugate

Unconjugated

Cat no : 12498-1-PBS

Print datasheet

Synonyms

验证数据展示

  • WB analysis using 12498-1-AP (same clone as 12498-1-PBS)

    WB analysis using 12498-1-AP (same clone as 12498-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 12498-1-AP (FOX2/RBM9 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12498-1-PBS in a different storage buffer formulation.

  • WB analysis using 12498-1-AP (same clone as 12498-1-PBS)

    WB analysis using 12498-1-AP (same clone as 12498-1-PBS)

    WB result of FOX2/RBM9 antibody (12498-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-FOX2/RBM9 transfected HEK-293 cells. This data was developed using the same antibody clone with 12498-1-PBS in a different storage buffer formulation.

  • IHC staining of human breast cancer using 12498-1-AP (same clone as 12498-1-PBS)

    IHC staining of human breast cancer using 12498-1-AP (same clone as 12498-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 12498-1-AP (FOX2/RBM9 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12498-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with FOX2/RBM9 antibody included
  • IF Staining of mouse brain using 12498-1-AP (same clone as 12498-1-PBS)

    IF Staining of mouse brain using 12498-1-AP (same clone as 12498-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using 12498-1-AP (FOX2/RBM9 antibody) at dilution of 1:100 and CoraLite488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 12498-1-PBS in a different storage buffer formulation.

  • WB analysis using 12498-1-AP (same clone as 12498-1-PBS)

    WB analysis using 12498-1-AP (same clone as 12498-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 12498-1-AP (FOX2/RBM9 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12498-1-PBS in a different storage buffer formulation.

  • IP experiment of HepG2 using 12498-1-AP (same clone as 12498-1-PBS)

    IP experiment of HepG2 using 12498-1-AP (same clone as 12498-1-PBS)

    IP result of anti-FOX2/RBM9 (IP:12498-1-AP, 4ug; Detection:12498-1-AP 1:1000) with HepG2 cells lysate 2680 ug. This data was developed using the same antibody clone with 12498-1-PBS in a different storage buffer formulation.

  • IP experiment of K-562 using 12498-1-AP (same clone as 12498-1-PBS)

    IP experiment of K-562 using 12498-1-AP (same clone as 12498-1-PBS)

    IP result of anti-FOX2/RBM9 (IP:12498-1-AP, 4ug; Detection:12498-1-AP 1:1000) with K-562 cells lysate 1760 ug. This data was developed using the same antibody clone with 12498-1-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 12498-1-AP (same clone as 12498-1-PBS)

    IF Staining of HepG2 using 12498-1-AP (same clone as 12498-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using FOX2/RBM9 antibody (12498-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red). This data was developed using the same antibody clone with 12498-1-PBS in a different storage buffer formulation.

Planning an IHC experiment? We recommend our IHCeasy FOX2/RBM9 Ready-To-Use IHC Kit. FOX2/RBM9 primary antibody included.

产品信息

12498-1-PBS targets FOX2/RBM9 in WB, IHC, IF/ICC, IF-P, IP, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IHC, IF/ICC, IF-P, IP, Indirect ELISA Application Description
Tested Reactivity human, mouse
Immunogen FOX2/RBM9 fusion protein Ag3168 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name RNA binding motif protein 9
Synonyms
Calculated Molecular Weight 390 aa, 41 kDa
Observed Molecular Weight 50 and 60 kDa
GenBank Accession NumberBC013115
Gene Symbol RBM9
Gene ID (NCBI) 23543
RRIDAB_2877861
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO43251
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

RBM9, also named as RBFOX2 and FOX2, is a RNA-binding protein that regulates alternative splicing events by binding to 5'-UGCAUGU-3' elements. There are several isoforms of RBM9 produced by variable use of multiple alternatively spliced internal exons and alternative splice sites. RBM9 may play an important role in tissue specific alternative splicing regulation.(PMID: 19688295, 21623373, 16537540)