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Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
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Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis using 12498-1-AP (same clone as 12498-1-PBS)

    WB analysis using 12498-1-AP (same clone as 12498-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 12498-1-AP (FOX2/RBM9 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12498-1-PBS in a different storage buffer formulation.

  • WB analysis using 12498-1-AP (same clone as 12498-1-PBS)

    WB analysis using 12498-1-AP (same clone as 12498-1-PBS)

    WB result of FOX2/RBM9 antibody (12498-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-FOX2/RBM9 transfected HEK-293 cells. This data was developed using the same antibody clone with 12498-1-PBS in a different storage buffer formulation.

  • IHC staining of human breast cancer using 12498-1-AP (same clone as 12498-1-PBS)

    IHC staining of human breast cancer using 12498-1-AP (same clone as 12498-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 12498-1-AP (FOX2/RBM9 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12498-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with FOX2/RBM9 antibody included
  • IF Staining of mouse brain using 12498-1-AP (same clone as 12498-1-PBS)

    IF Staining of mouse brain using 12498-1-AP (same clone as 12498-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using 12498-1-AP (FOX2/RBM9 antibody) at dilution of 1:100 and CoraLite488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 12498-1-PBS in a different storage buffer formulation.

  • WB analysis using 12498-1-AP (same clone as 12498-1-PBS)

    WB analysis using 12498-1-AP (same clone as 12498-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 12498-1-AP (FOX2/RBM9 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12498-1-PBS in a different storage buffer formulation.

  • IP experiment of HepG2 using 12498-1-AP (same clone as 12498-1-PBS)

    IP experiment of HepG2 using 12498-1-AP (same clone as 12498-1-PBS)

    IP result of anti-FOX2/RBM9 (IP:12498-1-AP, 4ug; Detection:12498-1-AP 1:1000) with HepG2 cells lysate 2680 ug. This data was developed using the same antibody clone with 12498-1-PBS in a different storage buffer formulation.

  • IP experiment of K-562 using 12498-1-AP (same clone as 12498-1-PBS)

    IP experiment of K-562 using 12498-1-AP (same clone as 12498-1-PBS)

    IP result of anti-FOX2/RBM9 (IP:12498-1-AP, 4ug; Detection:12498-1-AP 1:1000) with K-562 cells lysate 1760 ug. This data was developed using the same antibody clone with 12498-1-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 12498-1-AP (same clone as 12498-1-PBS)

    IF Staining of HepG2 using 12498-1-AP (same clone as 12498-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using FOX2/RBM9 antibody (12498-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red). This data was developed using the same antibody clone with 12498-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO验证

FOX2/RBM9 Polyclonal antibody, PBS Only

FOX2/RBM9 Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, IP, Indirect ELISA
Cat No. 12498-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, IF/ICC, IF-P, IP, Indirect ELISA

别名

FOX2, RBM9, HNRBP2, Hexaribonucleotide-binding protein 2, fxh

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy FOX2/RBM9 Ready-To-Use IHC Kit. FOX2/RBM9 primary antibody included.

产品信息

12498-1-PBS targets FOX2/RBM9 in WB, IHC, IF/ICC, IF-P, IP, Indirect ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IF/ICC, IF-P, IP, Indirect ELISA Application Description
经测试反应性 human, mouse
免疫原 FOX2/RBM9 fusion protein Ag3168 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 RNA binding motif protein 9
别名 FOX2, RBM9, HNRBP2, Hexaribonucleotide-binding protein 2, fxh
计算分子量 390 aa, 41 kDa
观测分子量 50 and 60 kDa
GenBank蛋白编号BC013115
基因名称 RBM9
Gene ID (NCBI) 23543
RRIDAB_2877861
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDO43251
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

RBM9, also named as RBFOX2 and FOX2, is a RNA-binding protein that regulates alternative splicing events by binding to 5'-UGCAUGU-3' elements. There are several isoforms of RBM9 produced by variable use of multiple alternatively spliced internal exons and alternative splice sites. RBM9 may play an important role in tissue specific alternative splicing regulation.(PMID: 19688295, 21623373, 16537540)