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验证数据展示

  • WB analysis using CL750-82997

    WB analysis using CL750-82997

    Various lysates were subjected to SDS PAGE followed by western blot with CL750-82997 (FOXO1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • FC experiment of MOLT-4 using CL750-82997

    FC experiment of MOLT-4 using CL750-82997

    1x10^6 MOLT-4 cells were intracellularly stained with 0.4 ug CoraLite® Plus 750-conjugated FOXO1 Recombinant antibody (CL750-82997, Clone:230183D1)(red), or 0.4 ug Isotype Control (CL750-30000) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

CoraLite® Plus 750-conjugated FOXO1 Recombinant antibody

FOXO1 Recombinant Antibody for WB, FC (Intra)
Cat No. CL750-82997

Print datasheet

CloneNo. 230183D1

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, FC (Intra)

Synonyms

Forkhead box protein O1, forkhead box O1, FKHR, FKH1, FKH 1

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite® Plus 750
Size: 

-/ -

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经过测试的应用

Positive WB detected inHEK-293 cells, HepG2 cells
Positive FC (Intra) detected inMOLT-4 cells
Planning an IHC experiment? We recommend our IHCeasy FOXO1 Ready-To-Use IHC Kit. FOXO1 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 82997-1-PBS

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL750-82997 targets FOXO1 in WB, FC (Intra) applications and shows reactivity with human samples.

Tested Applications WB, FC (Intra) Application Description
Tested Reactivity human
Immunogen FOXO1 fusion protein Ag13296 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name forkhead box O1
Synonyms Forkhead box protein O1, forkhead box O1, FKHR, FKH1, FKH 1
Calculated Molecular Weight 655 aa, 70 kDa
Observed Molecular Weight 70-80 kDa
GenBank Accession NumberBC021981
Gene Symbol FOXO1
Gene ID (NCBI) 2308
RRIDAB_3673761
Conjugate CoraLite® Plus 750 Fluorescent Dye
Excitation/Emission Maxima Wavelengths755 nm / 780 nm
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ12778
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

FOXO1, also named as FOXO1A, FKHR and FKH1, is a member of the FOXO subfamily of Forkhead transcription factors. FOXO1 is a transcription factor which acts as a regulator of cell responses to oxidative stress. FOXO1 interacts with LRPPRC and SIRT1. In the presence of KIRT1, FOXO1 mediates down-regulation of cyclin D1 and up-regulation of CDKN1B levels which are required for cell transition from proliferative growth to quiescence. FOXO1 contains three predicted protein kinase B phosphorylation sites (Thr-24, Ser-256, and Ser-319) that are conserved in other FOXO proteins. The t(2;13) and the variant t(1;13) translocations generate PAX3/FKHR and PAX7/FKHR fusion proteins respectively. The resulting protein is a transcriptional activator. Defects in FOXO1 are a cause of rhabdomyosarcoma type 2 (RMS2).

实验方案

Product Specific Protocols
WB protocol for CL Plus 750 FOXO1 antibody CL750-82997Download protocol
Standard Protocols
Click here to view our Standard Protocols