• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis using CL750-82997

    WB analysis using CL750-82997

    Various lysates were subjected to SDS PAGE followed by western blot with CL750-82997 (FOXO1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • FC experiment of MOLT-4 using CL750-82997

    FC experiment of MOLT-4 using CL750-82997

    1x10^6 MOLT-4 cells were intracellularly stained with 0.4 ug CoraLite® Plus 750-conjugated FOXO1 Recombinant antibody (CL750-82997, Clone:230183D1)(red), or 0.4 ug Isotype Control (CL750-30000) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

CoraLite® Plus 750-conjugated FOXO1 Recombinant antibody

FOXO1 Recombinant Antibody for WB, FC (Intra)
Cat No. CL750-82997

产品说明书

CloneNo. 230183D1

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, FC (Intra)

别名

Forkhead box protein O1, forkhead box O1, FKHR, FKH1, FKH 1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 750
规格: 

-/ -

点击咨询售前客服

点击咨询售后客服

经过测试的应用

Positive WB detected inHEK-293 cells, HepG2 cells
Positive FC (Intra) detected inMOLT-4 cells
Planning an IHC experiment? We recommend our IHCeasy FOXO1 Ready-To-Use IHC Kit. FOXO1 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 82997-1-PBS

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL750-82997 targets FOXO1 in WB, FC (Intra) applications and shows reactivity with human samples.

经测试应用 WB, FC (Intra) Application Description
经测试反应性 human
免疫原 FOXO1 fusion protein Ag13296 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 forkhead box O1
别名 Forkhead box protein O1, forkhead box O1, FKHR, FKH1, FKH 1
计算分子量 655 aa, 70 kDa
观测分子量 70-80 kDa
GenBank蛋白编号BC021981
基因名称 FOXO1
Gene ID (NCBI) 2308
RRIDAB_3673761
偶联类型 CoraLite® Plus 750 Fluorescent Dye
最大激发/发射波长755 nm / 780 nm
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ12778
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

FOXO1, also named as FOXO1A, FKHR and FKH1, is a member of the FOXO subfamily of Forkhead transcription factors. FOXO1 is a transcription factor which acts as a regulator of cell responses to oxidative stress. FOXO1 interacts with LRPPRC and SIRT1. In the presence of KIRT1, FOXO1 mediates down-regulation of cyclin D1 and up-regulation of CDKN1B levels which are required for cell transition from proliferative growth to quiescence. FOXO1 contains three predicted protein kinase B phosphorylation sites (Thr-24, Ser-256, and Ser-319) that are conserved in other FOXO proteins. The t(2;13) and the variant t(1;13) translocations generate PAX3/FKHR and PAX7/FKHR fusion proteins respectively. The resulting protein is a transcriptional activator. Defects in FOXO1 are a cause of rhabdomyosarcoma type 2 (RMS2).

实验方案

Product Specific Protocols
WB protocol for CL Plus 750 FOXO1 antibody CL750-82997Download protocol
Standard Protocols
Click here to view our Standard Protocols