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验证数据展示

  • FC experiment of Neuro-2a using CL488-67081

    FC experiment of Neuro-2a using CL488-67081

    1X10^6 Neuro-2a cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human FRZB (CL488-67081, Clone:2B4A1) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

CoraLite® Plus 488-conjugated FRZB Monoclonal antibody

FRZB Monoclonal Antibody for FC (Intra)
Cat No. CL488-67081

产品说明书

CloneNo. 2B4A1

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse

应用

FC (Intra)

别名

Frizzled related protein 1, FRITZ, Frezzled, FRE, FIZ

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive FC (Intra) detected inNeuro-2a cells
For other applications, we recommend the unconjugated version of this antibody, 67081-1-Ig

推荐稀释比

应用推荐稀释比
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-67081 targets FRZB in FC (Intra) applications and shows reactivity with human, mouse samples.

经测试应用 FC (Intra) Application Description
经测试反应性 human, mouse
免疫原 FRZB fusion protein Ag28147 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 frizzled-related protein
别名 Frizzled related protein 1, FRITZ, Frezzled, FRE, FIZ
计算分子量 325 aa, 36 kDa
观测分子量 36 kDa
GenBank蛋白编号BC027855
基因名称 FRZB
Gene ID (NCBI) 2487
RRIDAB_3084306
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式 Liquid
纯化方式Protein G purification
UNIPROT IDQ92765
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Frzb (also known as sFRP3) is a member of the secreted frizzled related protein (SFRP) family, originally identified as a chondrogenic factor during bone morphogenesis (PMID: 29209231). It was subsequently shown to be a regulator in Wnt signaling, and also have a role in regulating cell growth and differentiation in specific cell types. FRZB was seen to be acting as an oncogene in some cancer, such as metastatic renal cancer.

实验方案

Product Specific Protocols
FC protocol for CL Plus 488 FRZB antibody CL488-67081Download protocol
Standard Protocols
Click here to view our Standard Protocols