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验证数据展示

  • FC experiment of Neuro-2a using CL488-67081

    FC experiment of Neuro-2a using CL488-67081

    1X10^6 Neuro-2a cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human FRZB (CL488-67081, Clone:2B4A1) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

CoraLite® Plus 488-conjugated FRZB Monoclonal antibody

FRZB Monoclonal Antibody for FC (Intra)
Cat No. CL488-67081
CloneNo. 2B4A1

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse

Applications

FC (Intra)

Synonyms

Frizzled related protein 1, FRITZ, Frezzled, FRE, FIZ

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite® Plus 488
Size: 

-/ -

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经过测试的应用

Positive FC (Intra) detected inNeuro-2a cells
For other applications, we recommend the unconjugated version of this antibody, 67081-1-Ig

推荐稀释比

ApplicationDilution
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-67081 targets FRZB in FC (Intra) applications and shows reactivity with human, mouse samples.

Tested Applications FC (Intra) Application Description
Tested Reactivity human, mouse
Immunogen FRZB fusion protein Ag28147 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name frizzled-related protein
Synonyms Frizzled related protein 1, FRITZ, Frezzled, FRE, FIZ
Calculated Molecular Weight 325 aa, 36 kDa
Observed Molecular Weight 36 kDa
GenBank Accession NumberBC027855
Gene Symbol FRZB
Gene ID (NCBI) 2487
RRIDAB_3084306
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ92765
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Frzb (also known as sFRP3) is a member of the secreted frizzled related protein (SFRP) family, originally identified as a chondrogenic factor during bone morphogenesis (PMID: 29209231). It was subsequently shown to be a regulator in Wnt signaling, and also have a role in regulating cell growth and differentiation in specific cell types. FRZB was seen to be acting as an oncogene in some cancer, such as metastatic renal cancer.

实验方案

Product Specific Protocols
FC protocol for CL Plus 488 FRZB antibody CL488-67081Download protocol
Standard Protocols
Click here to view our Standard Protocols