验证数据展示
经过测试的应用
Positive WB detected in | LNCaP cells, 4T1 cells, MKN-45 cells, SGC-7901 cells |
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:5000-1:50000 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
发表文章中的应用
WB | See 2 publications below |
产品信息
67081-1-Ig targets FRZB in WB, ELISA applications and shows reactivity with Human, mouse samples.
Tested Applications | WB, ELISA Application Description |
Cited Applications | WB |
Tested Reactivity | Human, mouse |
Cited Reactivity | human |
Immunogen | FRZB fusion protein Ag28147 种属同源性预测 |
Host / Isotype | Mouse / IgG1 |
Class | Monoclonal |
Type | Antibody |
Full Name | frizzled-related protein |
Synonyms | FIZ, FRE, Frezzled, FRITZ, frizzled related protein, Frizzled related protein 1, FRP, FRP 3, FRZB, FRZB 1, FRZB PEN, FRZB1, FZRB, hFIZ, sFRP 3, SFRP3, SRFP3 |
Calculated Molecular Weight | 325 aa, 36 kDa |
Observed Molecular Weight | 36 kDa |
GenBank Accession Number | BC027855 |
Gene Symbol | FRZB |
Gene ID (NCBI) | 2487 |
RRID | AB_2882389 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein G purification |
UNIPROT ID | Q92765 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Frzb (also known as sFRP3) is a member of the secreted frizzled related protein (SFRP) family, originally identified as a chondrogenic factor during bone morphogenesis (PMID: 29209231). It was subsequently shown to be a regulator in Wnt signaling, and also have a role in regulating cell growth and differentiation in specific cell types. FRZB was seen to be acting as an oncogene in some cancer, such as metastatic renal cancer.
实验方案
Product Specific Protocols | |
---|---|
WB protocol for FRZB antibody 67081-1-Ig | Download protocol |
Standard Protocols | |
---|---|
Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Eur J Pharmacol Eltrombopag restores proliferative capacity and adipose-osteogenic balance of mesenchymal stromal cells in low-risk myelodysplastic syndromes | ||
Stem Cells Dev Chlorogenic Acid Promotes Osteogenic Differentiation of Human Dental Pulp Stem Cells Through Wnt Signaling. |