• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

FTO Recombinant antibody, PBS Only

FTO Recombinant Antibody for WB,Indirect ELISA,IHC,IF

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB,Indirect ELISA,IHC,IF

Conjugate

Unconjugated

CloneNo.

4L4

Cat No : 81471-1-PBS

Print datasheet

Synonyms

FTO, KIAA1752

验证数据展示

  • WB analysis using 81471-1-RR (same clone as 81471-1-PBS)

    WB analysis using 81471-1-RR (same clone as 81471-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 81471-1-RR (FTO antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 81471-1-PBS in a different storage buffer formulation.

  • IHC staining of human liver cancer using 81471-1-RR (same clone as 81471-1-PBS)

    IHC staining of human liver cancer using 81471-1-RR (same clone as 81471-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 81471-1-RR (FTO antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 81471-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with FTO antibody included
  • IF Staining of HepG2 using 81471-1-RR (same clone as 81471-1-PBS)

    IF Staining of HepG2 using 81471-1-RR (same clone as 81471-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using FTO antibody (81471-1-RR, Clone: 4L4 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 81471-1-PBS in a different storage buffer formulation.

Planning an IHC experiment? We recommend our IHCeasy FTO Ready-To-Use IHC Kit. FTO primary antibody included.

产品信息

81471-1-PBS targets FTO in WB, IF, IHC, Indirect ELISA applications and shows reactivity with Human samples.

Tested Applications WB,Indirect ELISA,IHC,IF Application Description
Tested Reactivity Human
Immunogen FTO fusion protein Ag26095 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name fat mass and obesity associated
Synonyms FTO, KIAA1752
Calculated Molecular Weight 58 kDa
Observed Molecular Weight58 kDa
GenBank Accession NumberNM_001080432
Gene Symbol FTO
Gene ID (NCBI) 79068
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ9C0B1
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

背景介绍

Fat mass and obesity-associated protein (FTO) has efficient oxidative demethylation activity targeting the abundant N6-methyladenosine (m6A) residues in RNA in vitro. Variants in the FTO (fat mass and obesity associated) gene are associated with increased body mass index in humans.