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FTO Recombinant antibody, PBS Only

FTO Uni-rAbTM Recombinant Antibody for WB, IHC, IF/ICC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

4L4

Cat no : 81471-1-PBS

Print datasheet

Synonyms

FTO, KIAA1752

验证数据展示

  • WB analysis using 81471-1-RR (same clone as 81471-1-PBS)

    WB analysis using 81471-1-RR (same clone as 81471-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 81471-1-RR (FTO antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 81471-1-PBS in a different storage buffer formulation.

  • IHC staining of human liver cancer using 81471-1-RR (same clone as 81471-1-PBS)

    IHC staining of human liver cancer using 81471-1-RR (same clone as 81471-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 81471-1-RR (FTO antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 81471-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with FTO antibody included
  • IF Staining of HepG2 using 81471-1-RR (same clone as 81471-1-PBS)

    IF Staining of HepG2 using 81471-1-RR (same clone as 81471-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using FTO antibody (81471-1-RR, Clone: 4L4 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 81471-1-PBS in a different storage buffer formulation.

Planning an IHC experiment? We recommend our IHCeasy FTO Ready-To-Use IHC Kit. FTO primary antibody included.

产品信息

81471-1-PBS targets FTO in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with Human samples.

Tested Applications WB, IHC, IF/ICC, Indirect ELISA Application Description
Tested Reactivity Human
Immunogen FTO fusion protein Ag26095 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name fat mass and obesity associated
Synonyms FTO, KIAA1752
Calculated Molecular Weight 58 kDa
Observed Molecular Weight58 kDa
GenBank Accession NumberNM_001080432
Gene Symbol FTO
Gene ID (NCBI) 79068
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ9C0B1
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Fat mass and obesity-associated protein (FTO) has efficient oxidative demethylation activity targeting the abundant N6-methyladenosine (m6A) residues in RNA in vitro. Variants in the FTO (fat mass and obesity associated) gene are associated with increased body mass index in humans.