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验证数据展示

  • WB analysis using 81471-1-RR

    WB analysis using 81471-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 81471-1-RR (FTO antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • IHC staining of human liver cancer using 81471-1-RR

    IHC staining of human liver cancer using 81471-1-RR

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 81471-1-RR (FTO antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with FTO antibody included
  • IF Staining of HepG2 using 81471-1-RR

    IF Staining of HepG2 using 81471-1-RR

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using FTO antibody (81471-1-RR, Clone: 4L4 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

  • FC experiment of NIH/3T3 using 81471-1-RR

    FC experiment of NIH/3T3 using 81471-1-RR

    1x10^6 NIH/3T3 cells were intracellularly stained with 0.25 ug FTO Recombinant antibody (81471-1-RR, Clone:4L4) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

FTO Recombinant antibody

FTO Uni-rAbTM Recombinant Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 81471-1-RR

产品说明书

CloneNo. 4L4

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, IF/ICC, ELISA

别名

4L4, KIAA1752

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHeLa cells, HEK-293T cells, LNCaP cells, Jurkat cells
Positive IHC detected inhuman liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells
Positive FC (Intra) detected inNIH/3T3 cells
Planning an IHC experiment? We recommend our IHCeasy FTO Ready-To-Use IHC Kit. FTO primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

81471-1-RR targets FTO in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 human, mouse
免疫原 FTO fusion protein Ag26095 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 fat mass and obesity associated
别名 4L4, KIAA1752
计算分子量 58 kDa
观测分子量58 kDa
GenBank蛋白编号NM_001080432
基因名称 FTO
Gene ID (NCBI) 79068
RRIDAB_2935551
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ9C0B1
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Fat mass and obesity-associated protein (FTO) has efficient oxidative demethylation activity targeting the abundant N6-methyladenosine (m6A) residues in RNA in vitro. Variants in the FTO (fat mass and obesity associated) gene are associated with increased body mass index in humans.

实验方案

Product Specific Protocols
WB protocol for FTO antibody 81471-1-RRDownload protocol
IHC protocol for FTO antibody 81471-1-RRDownload protocol
IF protocol for FTO antibody 81471-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols