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  • Featured Product
  • KD/KO Validated

FYN Monoclonal antibody, PBS Only

FYN Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1G4B1

Cat No : 66606-1-PBS

Print datasheet

Synonyms

FYN, p59 Fyn, Proto oncogene c Fyn, Proto oncogene Syn, SLK, Src like kinase, SYN, Tyrosine protein kinase Fyn

验证数据展示

  • WB analysis using 66606-1-Ig (same clone as 66606-1-PBS)

    WB analysis using 66606-1-Ig (same clone as 66606-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66606-1-Ig (FYN antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66606-1-PBS in a different storage buffer formulation.

  • WB analysis of HEK-293 using 66606-1-Ig (same clone as 66606-1-PBS)

    WB analysis of HEK-293 using 66606-1-Ig (same clone as 66606-1-PBS)

    WB result of FYN antibody (66606-1-Ig; 1:30000; incubated at room temperature for 1.5 hours) with sh-Control and sh-FYN transfected HEK-293 cells. This data was developed using the same antibody clone with 66606-1-PBS in a different storage buffer formulation.

  • IHC staining of human tonsillitis using 66606-1-Ig (same clone as 66606-1-PBS)

    IHC staining of human tonsillitis using 66606-1-Ig (same clone as 66606-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66606-1-Ig (FYN antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66606-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with FYN antibody included
  • IHC staining of human tonsillitis using 66606-1-Ig (same clone as 66606-1-PBS)

    IHC staining of human tonsillitis using 66606-1-Ig (same clone as 66606-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66606-1-Ig (FYN antibody) at dilution of 1:5000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66606-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with FYN antibody included
  • IF Staining of Neuro-2a using 66606-1-Ig (same clone as 66606-1-PBS)

    IF Staining of Neuro-2a using 66606-1-Ig (same clone as 66606-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed Neuro-2a cells using FYN antibody (66606-1-Ig, Clone: 1G4B1 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66606-1-PBS in a different storage buffer formulation.

Planning an IHC experiment? We recommend our IHCeasy FYN Ready-To-Use IHC Kit. FYN primary antibody included.

产品信息

66606-1-PBS targets FYN in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with Human, Mouse, Rat samples.

Tested Applications WB, IHC, IF/ICC, Indirect ELISA Application Description
Tested Reactivity Human, Mouse, Rat
Immunogen FYN fusion protein Ag11127 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name FYN oncogene related to SRC, FGR, YES
Synonyms FYN, p59 Fyn, Proto oncogene c Fyn, Proto oncogene Syn, SLK, Src like kinase, SYN, Tyrosine protein kinase Fyn
Calculated Molecular Weight537aa,61 kDa; 482aa,55 kDa
Observed Molecular Weight 60 kDa
GenBank Accession NumberBC032496
Gene Symbol FYN
Gene ID (NCBI) 2534
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP06241
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

FYN, also named as p59-Fyn and SLK, belongs to the protein kinase superfamily, Tyr protein kinase family and SRC subfamily. FYN is implicated in the control of cell growth. It plays a role in the regulation of intracellular calcium levels, with isoform 2 showing the greater ability to mobilize cytoplasmic calcium in comparison to isoform 1. FYN is required in brain development and mature brain function with important roles in the regulation of axon growth, axon guidance, and neurite extension. FYN blocks axon outgrowth and attraction induced by NTN1 by phosphorylating its receptor DDC.