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FYN Monoclonal antibody

FYN Monoclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 66606-1-Ig

产品说明书

CloneNo. 1G4B1

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, ELISA and More (1)

Proto oncogene Syn, Proto oncogene c Fyn, p59-Fyn, p59 Fyn, EC:2.7.10.2

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -


经过测试的应用

Positive WB detected inHEK-293 cells, Jurkat cells, Ramos cells, HeLa cells, MCF-7 cells, HSC-T6 cells, NIH/3T3 cells
Positive IHC detected inhuman tonsillitis tissue, human breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inNeuro-2a cells
Positive FC (Intra) detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy FYN Ready-To-Use IHC Kit. FYN primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite® Plus 647 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:30000
Immunohistochemistry (IHC)IHC : 1:2000-1:5000
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

66606-1-Ig targets FYN in WB, IHC, IF/ICC, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
文献引用应用WB, IHC, CoIP
经测试反应性 human, mouse, rat
文献引用反应性human, mouse, rat
免疫原 FYN fusion protein Ag11127 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 FYN oncogene related to SRC, FGR, YES
别名 Proto oncogene Syn, Proto oncogene c Fyn, p59-Fyn, p59 Fyn, EC:2.7.10.2
计算分子量537aa,61 kDa; 482aa,55 kDa
观测分子量 60 kDa
GenBank蛋白编号BC032496
基因名称 FYN
Gene ID (NCBI) 2534
RRIDAB_2881966
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein G purification
UNIPROT IDP06241
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

FYN, also named as p59-Fyn and SLK, belongs to the protein kinase superfamily, Tyr protein kinase family and SRC subfamily. FYN is implicated in the control of cell growth. It plays a role in the regulation of intracellular calcium levels, with isoform 2 showing the greater ability to mobilize cytoplasmic calcium in comparison to isoform 1. FYN is required in brain development and mature brain function with important roles in the regulation of axon growth, axon guidance, and neurite extension. FYN blocks axon outgrowth and attraction induced by NTN1 by phosphorylating its receptor DDC.

实验方案

Product Specific Protocols
WB protocol for FYN antibody 66606-1-IgDownload protocol
IHC protocol for FYN antibody 66606-1-IgDownload protocol
IF protocol for FYN antibody 66606-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

EBioMedicine

Obesity induces preadipocyte CD36 expression promoting inflammation via the disruption of lysosomal calcium homeostasis and lysosome function.

Authors - Xiaoxiao Luo
human,mouseIHC

EBioMedicine

Biallelic mutations in LAMA5 disrupts a skeletal noncanonical focal adhesion pathway and produces a distinct bent bone dysplasia.

Authors - Maya Barad
human

Inflammation

hPMSCs Regulate the Level of TNF-α and IL-10 in Th1 Cells and Improve Hepatic Injury in a GVHD Mouse Model via CD73/ADO/Fyn/Nrf2 Axis

Authors - Hengchao Zhang
humanWB

Biochem Pharmacol

PIKE-A promotes glioblastoma growth by driving PPP flux through increasing G6PD expression mediated by phosphorylation of STAT3.

Authors - Mingming Sun
humanIHC

Front Oncol

A four oxidative stress gene prognostic model and integrated immunity-analysis in pancreatic adenocarcinoma

Authors - Hao Wang
mouseWB

ACS Omega

Catalpol Promotes Osseointegration of Titanium Implants under Conditions of Type 2 Diabetes via AKT/GSK3β/FYN Pathway-Mediated NRF2 Activation

Authors - Yongzhi Zhang
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