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- KD/KO Validated
FYN Monoclonal antibody
FYN Monoclonal Antibody for WB, IHC, IF/ICC, ELISA
Host / Isotype
Mouse / IgG1
Reactivity
Human, Mouse, Rat and More (3)
Applications
WB, IHC, IF/ICC, ELISA
Conjugate
Unconjugated
CloneNo.
1G4B1
验证数据展示
经过测试的应用
| Positive WB detected in | HEK-293 cells, Jurkat cells, Ramos cells, HeLa cells, MCF-7 cells, HSC-T6 cells, NIH/3T3 cells |
| Positive IHC detected in | human tonsillitis tissue, human breast cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | Neuro-2a cells |
Planning an IHC experiment? We recommend our IHCeasy FYN Ready-To-Use IHC Kit. FYN primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite® Plus 647 conjugated versions of this antibody.
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:30000 |
| Immunohistochemistry (IHC) | IHC : 1:2000-1:5000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:400-1:1600 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 9 publications below |
| IHC | See 3 publications below |
产品信息
66606-1-Ig targets FYN in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human, Mouse, Rat samples.
| Tested Applications | WB, IHC, IF/ICC, ELISA Application Description |
| Cited Applications | WB, IHC |
| Tested Reactivity | Human, Mouse, Rat |
| Cited Reactivity | human, mouse, rat |
| Immunogen | FYN fusion protein Ag11127 种属同源性预测 |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Full Name | FYN oncogene related to SRC, FGR, YES |
| Synonyms | FYN, p59 Fyn, Proto oncogene c Fyn, Proto oncogene Syn, SLK, Src like kinase, SYN, Tyrosine protein kinase Fyn |
| Calculated Molecular Weight | 537aa,61 kDa; 482aa,55 kDa |
| Observed Molecular Weight | 60 kDa |
| GenBank Accession Number | BC032496 |
| Gene Symbol | FYN |
| Gene ID (NCBI) | 2534 |
| RRID | AB_2881966 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein G purification |
| UNIPROT ID | P06241 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
FYN, also named as p59-Fyn and SLK, belongs to the protein kinase superfamily, Tyr protein kinase family and SRC subfamily. FYN is implicated in the control of cell growth. It plays a role in the regulation of intracellular calcium levels, with isoform 2 showing the greater ability to mobilize cytoplasmic calcium in comparison to isoform 1. FYN is required in brain development and mature brain function with important roles in the regulation of axon growth, axon guidance, and neurite extension. FYN blocks axon outgrowth and attraction induced by NTN1 by phosphorylating its receptor DDC.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for FYN antibody 66606-1-Ig | Download protocol |
| IHC protocol for FYN antibody 66606-1-Ig | Download protocol |
| IF protocol for FYN antibody 66606-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
EBioMedicine Obesity induces preadipocyte CD36 expression promoting inflammation via the disruption of lysosomal calcium homeostasis and lysosome function. | ||
EBioMedicine Biallelic mutations in LAMA5 disrupts a skeletal noncanonical focal adhesion pathway and produces a distinct bent bone dysplasia. | ||
Biochem Pharmacol PIKE-A promotes glioblastoma growth by driving PPP flux through increasing G6PD expression mediated by phosphorylation of STAT3. | ||
Front Oncol A four oxidative stress gene prognostic model and integrated immunity-analysis in pancreatic adenocarcinoma | ||
Exp Cell Res ISG15 promotes esophageal squamous cell carcinoma tumorigenesis via c-MET/Fyn/β-catenin signaling pathway. | ||
Exp Cell Res Src-mediated Tyr353 phosphorylation of IP3R1 promotes its stability and causes apoptosis in palmitic acid-treated hepatocytes. |
