验证数据展示
经过测试的应用
Positive IF-P detected in | human tonsillitis tissue |
Positive IF/ICC detected in | Neuro-2a cells |
Positive FC (Intra) detected in | HeLa cells |
推荐稀释比
应用 | 推荐稀释比 |
---|---|
Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
发表文章中的应用
WB | See 1 publications below |
产品信息
CL488-66606 targets FYN in WB, IF/ICC, IF-P, FC (Intra) applications and shows reactivity with human, mouse, rat samples.
经测试应用 | IF/ICC, IF-P, FC (Intra) Application Description |
文献引用应用 | WB |
经测试反应性 | human, mouse, rat |
文献引用反应性 | mouse |
免疫原 | FYN fusion protein Ag11127 种属同源性预测 |
宿主/亚型 | Mouse / IgG1 |
抗体类别 | Monoclonal |
产品类型 | Antibody |
全称 | FYN oncogene related to SRC, FGR, YES |
别名 | Proto oncogene Syn, Proto oncogene c Fyn, p59-Fyn, p59 Fyn, EC:2.7.10.2 |
计算分子量 | 537aa,61 kDa; 482aa,55 kDa |
观测分子量 | 60 kDa |
GenBank蛋白编号 | BC032496 |
基因名称 | FYN |
Gene ID (NCBI) | 2534 |
RRID | AB_2883360 |
偶联类型 | CoraLite® Plus 488 Fluorescent Dye |
最大激发/发射波长 | 493 nm / 522 nm |
形式 | Liquid |
纯化方式 | Protein G purification |
UNIPROT ID | P06241 |
储存缓冲液 | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
储存条件 | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
Product Specific Protocols | |
---|---|
IF protocol for CL Plus 488 FYN antibody CL488-66606 | Download protocol |
FC protocol for CL Plus 488 FYN antibody CL488-66606 | Download protocol |
Standard Protocols | |
---|---|
Click here to view our Standard Protocols |