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验证数据展示

  • FC experiment of HeLa using CL647-66606

    FC experiment of HeLa using CL647-66606

    1X10^6 HeLa cells were intracellularly stained with 0.2 ug CoraLite® Plus 647 Anti-Human FYN (CL647-66606, Clone:1G4B1) (red), or 0.2 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Featured Product
  • KD/KO验证

CoraLite® Plus 647-conjugated FYN Monoclonal antibody

FYN Monoclonal Antibody for FC (Intra)
Cat No. CL647-66606

产品说明书

CloneNo. 1G4B1

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse, rat

应用

FC (Intra)

别名

Proto oncogene Syn, Proto oncogene c Fyn, p59-Fyn, p59 Fyn, EC:2.7.10.2

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 647
规格: 

-/ -

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经过测试的应用

Positive FC (Intra) detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy FYN Ready-To-Use IHC Kit. FYN primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 66606-1-Ig

推荐稀释比

应用推荐稀释比
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL647-66606 targets FYN in FC (Intra) applications and shows reactivity with human, mouse, rat samples.

经测试应用 FC (Intra) Application Description
经测试反应性 human, mouse, rat
免疫原 FYN fusion protein Ag11127 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 FYN oncogene related to SRC, FGR, YES
别名 Proto oncogene Syn, Proto oncogene c Fyn, p59-Fyn, p59 Fyn, EC:2.7.10.2
计算分子量537aa,61 kDa; 482aa,55 kDa
观测分子量 60 kDa
GenBank蛋白编号BC032496
基因名称 FYN
Gene ID (NCBI) 2534
RRIDAB_2935063
偶联类型 CoraLite® Plus 647 Fluorescent Dye
最大激发/发射波长654 nm / 674 nm
形式 Liquid
纯化方式Protein G purification
UNIPROT IDP06241
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

FYN, also named as p59-Fyn and SLK, belongs to the protein kinase superfamily, Tyr protein kinase family and SRC subfamily. FYN is implicated in the control of cell growth. It plays a role in the regulation of intracellular calcium levels, with isoform 2 showing the greater ability to mobilize cytoplasmic calcium in comparison to isoform 1. FYN is required in brain development and mature brain function with important roles in the regulation of axon growth, axon guidance, and neurite extension. FYN blocks axon outgrowth and attraction induced by NTN1 by phosphorylating its receptor DDC.

实验方案

Product Specific Protocols
FC protocol for CL Plus 647 FYN antibody CL647-66606Download protocol
Standard Protocols
Click here to view our Standard Protocols