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验证数据展示

  • IF Staining of human tonsillitis using CL594-66321

    IF Staining of human tonsillitis using CL594-66321

    Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using CL594-66321 (Fascin antibody) at dilution of 1:100.

  • IF Staining of human tonsillitis using CL594-66321

    IF Staining of human tonsillitis using CL594-66321

    Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using CL594-66321 (Fascin antibody) at dilution of 1:100.

  • FC experiment of HeLa using CL594-66321

    FC experiment of HeLa using CL594-66321

    1X10^6 HeLa cells were intracellularly stained with 0.2 ug CoraLite®594 Anti-Human Fascin (CL594-66321, Clone:1B5G3) (red), or 0.2 ug Mouse IgG2a Isotype Control (CL594-66360-2, Clone: K11A1B2A2) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Featured Product
  • KD/KO验证

CoraLite®594-conjugated Fascin Monoclonal antibody

Fascin Monoclonal Antibody for IF-P, FC (Intra)
Cat No. CL594-66321

产品说明书

CloneNo. 1B5G3

宿主/亚型

Mouse / IgG2a

种属反应性

human

应用

IF-P, FC (Intra)

别名

FSCN1, Fascin 1,Fascin, FAN1, 55 kDa actin-bundling protein, 55 kDa actin bundling protein

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite®594
规格: 

-/ -

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经过测试的应用

Positive IF-P detected inhuman tonsillitis tissue
Positive FC (Intra) detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy Fascin Ready-To-Use IHC Kit. Fascin primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 66321-1-Ig

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)-PIF-P : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL594-66321 targets Fascin in IF-P, FC (Intra) applications and shows reactivity with human samples.

经测试应用 IF-P, FC (Intra) Application Description
经测试反应性 human
免疫原 Fascin fusion protein Ag6074 种属同源性预测
宿主/亚型 Mouse / IgG2a
抗体类别 Monoclonal
产品类型 Antibody
全称 fascin homolog 1, actin-bundling protein (Strongylocentrotus purpuratus)
别名 FSCN1, Fascin 1,Fascin, FAN1, 55 kDa actin-bundling protein, 55 kDa actin bundling protein
计算分子量 55 kDa
GenBank蛋白编号BC000521
基因名称 Fascin
Gene ID (NCBI) 6624
ENSEMBL Gene IDENSG00000075618
RRIDAB_2883557
偶联类型 CoraLite®594 Fluorescent Dye
最大激发/发射波长588 nm / 604 nm
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ16658
储存缓冲液 PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Fascin is a highly conserved and ubiquitous actin cross-linking protein that has a major function in cell motility and adhesion. Fascin localizes to a number of highly dynamic cellular structures that require strong mechanical support which include stress fibers and cellular protrusions such as microvilli, microspikes and lamellipodia. Fascin is up-regulated in many human carcinomas. It's a marker for Reed-Sternberg cells in Hodgkin's disease; it is also a highly selective marker for dendritic cells of lymphoid tissues.

实验方案

Product Specific Protocols
IF protocol for CL594 Fascin antibody CL594-66321Download protocol
FC protocol for CL594 Fascin antibody CL594-66321Download protocol
Standard Protocols
Click here to view our Standard Protocols