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验证数据展示

  • FC experiment of HeLa using CL647-66321

    FC experiment of HeLa using CL647-66321

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite® Plus 647 Anti-Human Fascin (CL647-66321, Clone:1B5G3) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • IF Staining of human tonsillitis using CL647-66321

    IF Staining of human tonsillitis using CL647-66321

    Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using CoraLite® Plus 647 Fascin antibody (CL647-66321, Clone: 1B5G3 ) at dilution of 1:200.

  • Featured Product
  • KD/KO验证

CoraLite® Plus 647-conjugated Fascin Monoclonal antibody

Fascin Monoclonal Antibody for IF-P, FC (Intra)
Cat No. CL647-66321

产品说明书

CloneNo. 1B5G3

宿主/亚型

Mouse / IgG2a

种属反应性

human

应用

IF-P, FC (Intra)

别名

55 kDa actin bundling protein, FAN1, Fascin, Fascin 1,Fascin, FLJ38511, FSCN1, HSN, p55, Singed like protein, SNL

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 647
规格: 

-/ -

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经过测试的应用

Positive IF-P detected inhuman tonsillitis tissue
Positive FC (Intra) detected inHeLa cells
Positive FC detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy Fascin Ready-To-Use IHC Kit. Fascin primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 66321-1-Ig

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)-PIF-P : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL647-66321 targets Fascin in IF-P, FC (Intra) applications and shows reactivity with human samples.

经测试应用 IF-P, FC (Intra) Application Description
经测试反应性 human
免疫原 Fascin fusion protein Ag6074 种属同源性预测
宿主/亚型 Mouse / IgG2a
抗体类别 Monoclonal
产品类型 Antibody
全称 fascin homolog 1, actin-bundling protein (Strongylocentrotus purpuratus)
别名 55 kDa actin bundling protein, FAN1, Fascin, Fascin 1,Fascin, FLJ38511, FSCN1, HSN, p55, Singed like protein, SNL
计算分子量 55 kDa
观测分子量 55 kDa
GenBank蛋白编号BC000521
基因名称 Fascin
Gene ID (NCBI) 6624
ENSEMBL Gene IDENSG00000075618
RRIDAB_2920280
偶联类型 CoraLite® Plus 647 Fluorescent Dye
最大激发/发射波长654 nm / 674 nm
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ16658
储存缓冲液 PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Fascin is a highly conserved and ubiquitous actin cross-linking protein that has a major function in cell motility and adhesion. Fascin localizes to a number of highly dynamic cellular structures that require strong mechanical support which include stress fibers and cellular protrusions such as microvilli, microspikes and lamellipodia. Fascin is up-regulated in many human carcinomas. It's a marker for Reed-Sternberg cells in Hodgkin's disease; it is also a highly selective marker for dendritic cells of lymphoid tissues.

实验方案

Product Specific Protocols
IF protocol for CL Plus 647 Fascin antibody CL647-66321Download protocol
FC protocol for CL Plus 647 Fascin antibody CL647-66321Download protocol
Standard Protocols
Click here to view our Standard Protocols