- Featured Product
- KD/KO Validated
CoraLite® Plus 647-conjugated Fascin Monoclonal antibody
Fascin Monoclonal Antibody for IF-P, FC (Intra)
Host / Isotype
Mouse / IgG2a
Reactivity
human
Applications
IF-P, FC (Intra)
Conjugate
CoraLite® Plus 647 Fluorescent Dye
CloneNo.
1B5G3
验证数据展示
经过测试的应用
| Positive IF-P detected in | human tonsillitis tissue |
| Positive FC (Intra) detected in | HeLa cells |
| Positive FC detected in | HeLa cells |
Planning an IHC experiment? We recommend our IHCeasy Fascin Ready-To-Use IHC Kit. Fascin primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 66321-1-Ig
推荐稀释比
| Application | Dilution |
|---|---|
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
| Flow Cytometry (FC) | FC : 0.40 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL647-66321 targets Fascin in IF-P, FC (Intra) applications and shows reactivity with human samples.
| Tested Applications | IF-P, FC (Intra) Application Description |
| Tested Reactivity | human |
| Immunogen | Fascin fusion protein Ag6074 种属同源性预测 |
| Host / Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Full Name | fascin homolog 1, actin-bundling protein (Strongylocentrotus purpuratus) |
| Synonyms | 55 kDa actin bundling protein, FAN1, Fascin, Fascin 1,Fascin, FLJ38511, FSCN1, HSN, p55, Singed like protein, SNL |
| Calculated Molecular Weight | 55 kDa |
| Observed Molecular Weight | 55 kDa |
| GenBank Accession Number | BC000521 |
| Gene Symbol | Fascin |
| Gene ID (NCBI) | 6624 |
| ENSEMBL Gene ID | ENSG00000075618 |
| RRID | AB_2920280 |
| Conjugate | CoraLite® Plus 647 Fluorescent Dye |
| Excitation/Emission Maxima Wavelengths | 654 nm / 674 nm |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | Q16658 |
| Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Fascin is a highly conserved and ubiquitous actin cross-linking protein that has a major function in cell motility and adhesion. Fascin localizes to a number of highly dynamic cellular structures that require strong mechanical support which include stress fibers and cellular protrusions such as microvilli, microspikes and lamellipodia. Fascin is up-regulated in many human carcinomas. It's a marker for Reed-Sternberg cells in Hodgkin's disease; it is also a highly selective marker for dendritic cells of lymphoid tissues.
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for CL Plus 647 Fascin antibody CL647-66321 | Download protocol |
| FC protocol for CL Plus 647 Fascin antibody CL647-66321 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
