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Host
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Species Reactivity
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0
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验证数据展示

  • WB analysis using 20685-1-AP (same clone as 20685-1-PBS)

    WB analysis using 20685-1-AP (same clone as 20685-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 20685-1-AP (Filamin B antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20685-1-PBS in a different storage buffer formulation.

  • IHC staining of human liver cancer using 20685-1-AP (same clone as 20685-1-PBS)

    IHC staining of human liver cancer using 20685-1-AP (same clone as 20685-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 20685-1-AP (FLNB antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20685-1-PBS in a different storage buffer formulation.

  • IHC staining of human liver cancer using 20685-1-AP (same clone as 20685-1-PBS)

    IHC staining of human liver cancer using 20685-1-AP (same clone as 20685-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 20685-1-AP (FLNB antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20685-1-PBS in a different storage buffer formulation.

  • IHC staining of human colon using 20685-1-AP (same clone as 20685-1-PBS)

    IHC staining of human colon using 20685-1-AP (same clone as 20685-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human normal colon slide using 20685-1-AP (FLNB antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20685-1-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 20685-1-AP (same clone as 20685-1-PBS)

    IF Staining of HepG2 using 20685-1-AP (same clone as 20685-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using FLNB antibody (20685-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). This data was developed using the same antibody clone with 20685-1-PBS in a different storage buffer formulation.

Filamin B Polyclonal antibody, PBS Only

Filamin B Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 20685-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

FLNB, FilaminB, Filamin-3, Beta-filamin, Actin-binding-like protein

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

20685-1-PBS targets Filamin B in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 Peptide 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 filamin B, beta (actin binding protein 278)
别名 FLNB, FilaminB, Filamin-3, Beta-filamin, Actin-binding-like protein
计算分子量 278 kDa
观测分子量 250-278 kDa
GenBank蛋白编号NM_001457
基因名称 FLNB
Gene ID (NCBI) 2317
RRIDAB_10863658
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDO75369
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

FLNB, also named as FLN1L, FLN3, TABP, TAP and Fh1, belongs to the filamin family. FLNB connects cell membrane constituents to the actin cytoskeleton. It may promote orthogonal branching of actin filaments and links actin filaments to membrane glycoproteins. It is an anchors various transmembrane proteins to the actin cytoskeleton. Interaction with FLNA, FLNB may allow neuroblast migration from the ventricular zone into the cortical plate. Various interactions and localizations of isoforms affect myotube morphology and myogenesis. The antibody is specific to FLNB. It can recognize isoform1,2,3,6,7 of FLNB. It has no cross reaction to FLNA and FLNC.