DDDDK tag Monoclonal antibody (Binds to FLAG® tag epitope)
DDDDK tag Monoclonal Antibody for ELISA
Host / Isotype
Mouse / IgG2b
Reactivity
human, mouse, rat
Applications
ELISA and More (4)
Conjugate
Unconjugated
CloneNo.
2C3F4
验证数据展示
Planning an IF experiment? We recommend our CoraLite® Plus 488, CoraLite®594 and CoraLite® Plus 647 conjugated versions of this antibody.
产品信息
66008-1-Ig targets DDDDK tag in WB, IF, IP, RIP, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Applications | ELISA Application Description |
Cited Applications | WB, IF, IP, RIP |
Tested Reactivity | human, mouse, rat |
Cited Reactivity | human, mouse |
Immunogen | Peptide 种属同源性预测 |
Host / Isotype | Mouse / IgG2b |
Class | Monoclonal |
Type | Antibody |
Full Name | DDDDK tag |
Synonyms | |
Gene Symbol | |
Gene ID (NCBI) | |
RRID | AB_2881491 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein G purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
DDDDK tag, also known as DDK tag, is a hydrophilic tag for recombinant protein technology with the sequence DYKDDDDK. The structure of DDDDK tag has been optimized for compatibility with the proteins it is attached to and unlikely to denature or inactivated them. For its high hydrophilic nature, the DDDDK tag is likely to be located on the surface of a fusion protein, which enables the tag more likely to be accessible to antibodies. This product is generated against 1xDDDDK tag (DYKDDDDK) and can recognize protein containing one or more DDDDK tags, no matter it is/they are located at N-terminal, C-terminal or at internal regions. The binding of this product with DDDDK tagged fusion protein does not depend on calcium ion. The clone of this monoclonal antibody hybridoma is 2C3F4, which is different from any other vendors.
实验方案
Product Specific Protocols | |
---|---|
IF protocol for DDDDK tag antibody 66008-1-Ig | Download protocol |
IP protocol for DDDDK tag antibody 66008-1-Ig | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
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