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CoraLite® Plus 488-conjugated Flotillin 1 Monoclonal antibody

Flotillin 1 Monoclonal Antibody for FC (Intra)

Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse, rat, pig, rabbit

Applications

FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

1D6C6

Cat no : CL488-67968

Print datasheet

Synonyms

FLOT1, Flotillin-1, 1D6C6

验证数据展示

  • FC experiment of Raji using CL488-67968

    FC experiment of Raji using CL488-67968

    1x10^6 Raji cells were intracellularly stained with 0.8 ug CoraLite® Plus 488-conjugated Flotillin 1 Monoclonal antibody (CL488-67968, Clone:1D6C6)(red), or 0.8 ug CoraLite® Plus 488 Mouse IgG2b isotype control (11B8C4) (CL488-66360-3, Clone: 11B8C4) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

经过测试的应用

Positive FC (Intra) detected inRaji cells
Planning an IHC experiment? We recommend our IHCeasy FLOT1 Ready-To-Use IHC Kit. FLOT1 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 67968-1-Ig

推荐稀释比

ApplicationDilution
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-67968 targets Flotillin 1 in FC (Intra) applications and shows reactivity with human, mouse, rat, pig, rabbit samples.

Tested Applications FC (Intra) Application Description
Tested Reactivity human, mouse, rat, pig, rabbit
Immunogen Flotillin 1 fusion protein Ag8694 种属同源性预测
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Full Name flotillin 1
Synonyms FLOT1, Flotillin-1, 1D6C6
Calculated Molecular Weight 47 kDa
Observed Molecular Weight 47 kDa
GenBank Accession NumberBC001146
Gene Symbol Flotillin 1
Gene ID (NCBI) 10211
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein A purification
UNIPROT IDO75955
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Flotillins are membrane-associated proteins that contain two homologous isoforms, flotillin 1 (FLOT1) and flotillin 2 (FLOT2). They are highly conserved and widely expressed proteins that commonly used as markers of lipid rafts. Flotillins are involved in various signaling pathways, cell adhesion, membrane trafficking and axonal growth.

实验方案

Product Specific Protocols
FC protocol for CL Plus 488 Flotillin 1 antibody CL488-67968Download protocol
Standard Protocols
Click here to view our Standard Protocols