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验证数据展示

  • WB analysis using 67968-1-Ig

    WB analysis using 67968-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67968-1-Ig (Flotillin 1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • IHC staining of human ovary tumor using 67968-1-Ig

    IHC staining of human ovary tumor using 67968-1-Ig

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 67968-1-Ig (Flotillin 1 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with FLOT1 antibody included
  • FC experiment of Raji using 67968-1-Ig

    FC experiment of Raji using 67968-1-Ig

    1x10^6 Raji cells were intracellularly stained with 0.2 ug Flotillin 1 Monoclonal antibody (67968-1-Ig, Clone:1D6C6) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1)(red), or 0.2 ug Mouse IgG2b isotype control Mouse McAb (66360-3-Ig, Clone: 11B8C4) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • IHC staining of human ovary tumor using 67968-1-Ig

    IHC staining of human ovary tumor using 67968-1-Ig

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 67968-1-Ig (Flotillin 1 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with FLOT1 antibody included

Flotillin 1 Monoclonal antibody

Flotillin 1 Monoclonal Antibody for WB, FC (Intra), ELISA
Cat No. 67968-1-Ig

产品说明书

CloneNo. 1D6C6

宿主/亚型

Mouse / IgG2b

种属反应性

human, mouse, rat, pig, rabbit

应用

WB, FC (Intra), ELISA

别名

FLOT1, Flotillin-1, 1D6C6

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive WB detected inLNCaP cells, Raji cells, Jurkat cells, MOLT-4 cells, human peripheral blood platelets, pig brain tissue, rabbit brain tissue, rat brain tissue, mouse brain tissue
Positive FC (Intra) detected inRaji cells
Planning an IHC experiment? We recommend our IHCeasy FLOT1 Ready-To-Use IHC Kit. FLOT1 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67968-1-Ig targets Flotillin 1 in WB, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat, pig, rabbit samples.

经测试应用 WB, FC (Intra), ELISA Application Description
经测试反应性 human, mouse, rat, pig, rabbit
免疫原 Flotillin 1 fusion protein Ag8694 种属同源性预测
宿主/亚型 Mouse / IgG2b
抗体类别 Monoclonal
产品类型 Antibody
全称 flotillin 1
别名 FLOT1, Flotillin-1, 1D6C6
计算分子量 47 kDa
观测分子量 47 kDa
GenBank蛋白编号BC001146
基因名称 Flotillin 1
Gene ID (NCBI) 10211
RRIDAB_2918719
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDO75955
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Flotillins are membrane-associated proteins that contain two homologous isoforms, flotillin 1 (FLOT1) and flotillin 2 (FLOT2). They are highly conserved and widely expressed proteins that commonly used as markers of lipid rafts. Flotillins are involved in various signaling pathways, cell adhesion, membrane trafficking and axonal growth.

实验方案

Product Specific Protocols
WB protocol for Flotillin 1 antibody 67968-1-IgDownload protocol
IHC protocol for Flotillin 1 antibody 67968-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols