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Flotillin 1 Monoclonal antibody, PBS Only

Flotillin 1 Monoclonal Antibody for WB, FC (Intra), Indirect ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse, rat, pig, rabbit

Applications

WB, FC (Intra), Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1D6C6

Cat no : 67968-1-PBS

Print datasheet

Synonyms

FLOT1, Flotillin-1, 1D6C6

验证数据展示

  • WB analysis using 67968-1-Ig (same clone as 67968-1-PBS)

    WB analysis using 67968-1-Ig (same clone as 67968-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67968-1-Ig (Flotillin 1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67968-1-PBS in a different storage buffer formulation.

  • IHC staining of human ovary tumor using 67968-1-Ig (same clone as 67968-1-PBS)

    IHC staining of human ovary tumor using 67968-1-Ig (same clone as 67968-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 67968-1-Ig (Flotillin 1 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67968-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with FLOT1 antibody included
  • FC experiment of Raji using 67968-1-Ig (same clone as 67968-1-PBS)

    FC experiment of Raji using 67968-1-Ig (same clone as 67968-1-PBS)

    1x10^6 Raji cells were intracellularly stained with 0.2 ug Flotillin 1 Monoclonal antibody (67968-1-Ig, Clone:1D6C6) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1)(red), or 0.2 ug Mouse IgG2b isotype control Mouse McAb (66360-3-Ig, Clone: 11B8C4) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 67968-1-PBS in a different storage buffer formulation.

  • IHC staining of human ovary tumor using 67968-1-Ig (same clone as 67968-1-PBS)

    IHC staining of human ovary tumor using 67968-1-Ig (same clone as 67968-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 67968-1-Ig (Flotillin 1 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67968-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with FLOT1 antibody included
Planning an IHC experiment? We recommend our IHCeasy FLOT1 Ready-To-Use IHC Kit. FLOT1 primary antibody included.

产品信息

67968-1-PBS targets Flotillin 1 in WB, FC (Intra), Indirect ELISA applications and shows reactivity with human, mouse, rat, pig, rabbit samples.

Tested Applications WB, FC (Intra), Indirect ELISA Application Description
Tested Reactivity human, mouse, rat, pig, rabbit
Immunogen Flotillin 1 fusion protein Ag8694 种属同源性预测
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Full Name flotillin 1
Synonyms FLOT1, Flotillin-1, 1D6C6
Calculated Molecular Weight 47 kDa
Observed Molecular Weight 47 kDa
GenBank Accession NumberBC001146
Gene Symbol Flotillin 1
Gene ID (NCBI) 10211
RRIDAB_2918719
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDO75955
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Flotillins are membrane-associated proteins that contain two homologous isoforms, flotillin 1 (FLOT1) and flotillin 2 (FLOT2). They are highly conserved and widely expressed proteins that commonly used as markers of lipid rafts. Flotillins are involved in various signaling pathways, cell adhesion, membrane trafficking and axonal growth.