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Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis using 67968-1-Ig (same clone as 67968-1-PBS)

    WB analysis using 67968-1-Ig (same clone as 67968-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67968-1-Ig (Flotillin 1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67968-1-PBS in a different storage buffer formulation.

  • IHC staining of human ovary tumor using 67968-1-Ig (same clone as 67968-1-PBS)

    IHC staining of human ovary tumor using 67968-1-Ig (same clone as 67968-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 67968-1-Ig (Flotillin 1 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67968-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with FLOT1 antibody included
  • FC experiment of Raji using 67968-1-Ig (same clone as 67968-1-PBS)

    FC experiment of Raji using 67968-1-Ig (same clone as 67968-1-PBS)

    1x10^6 Raji cells were intracellularly stained with 0.2 ug Flotillin 1 Monoclonal antibody (67968-1-Ig, Clone:1D6C6) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1)(red), or 0.2 ug Mouse IgG2b isotype control Mouse McAb (66360-3-Ig, Clone: 11B8C4) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 67968-1-PBS in a different storage buffer formulation.

  • IHC staining of human ovary tumor using 67968-1-Ig (same clone as 67968-1-PBS)

    IHC staining of human ovary tumor using 67968-1-Ig (same clone as 67968-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 67968-1-Ig (Flotillin 1 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67968-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with FLOT1 antibody included

Flotillin 1 Monoclonal antibody, PBS Only

Flotillin 1 Monoclonal Antibody for WB, FC (Intra), Indirect ELISA
Cat No. 67968-1-PBS

产品说明书

CloneNo. 1D6C6

宿主/亚型

Mouse / IgG2b

种属反应性

human, mouse, rat, pig, rabbit

应用

WB, FC (Intra), Indirect ELISA

别名

FLOT1, Flotillin-1, 1D6C6

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy FLOT1 Ready-To-Use IHC Kit. FLOT1 primary antibody included.

产品信息

67968-1-PBS targets Flotillin 1 in WB, FC (Intra), Indirect ELISA applications and shows reactivity with human, mouse, rat, pig, rabbit samples.

经测试应用 WB, FC (Intra), Indirect ELISA Application Description
经测试反应性 human, mouse, rat, pig, rabbit
免疫原 Flotillin 1 fusion protein Ag8694 种属同源性预测
宿主/亚型 Mouse / IgG2b
抗体类别 Monoclonal
产品类型 Antibody
全称 flotillin 1
别名 FLOT1, Flotillin-1, 1D6C6
计算分子量 47 kDa
观测分子量 47 kDa
GenBank蛋白编号BC001146
基因名称 Flotillin 1
Gene ID (NCBI) 10211
RRIDAB_2918719
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDO75955
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Flotillins are membrane-associated proteins that contain two homologous isoforms, flotillin 1 (FLOT1) and flotillin 2 (FLOT2). They are highly conserved and widely expressed proteins that commonly used as markers of lipid rafts. Flotillins are involved in various signaling pathways, cell adhesion, membrane trafficking and axonal growth.